Cell Culture Vessel Geometry and Flow Path for Closed iPS Culture

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Solution Overview

Problem

Existing technologies lack an efficient device for culturing induced pluripotent stem cells (iPS cells) and other cells without immunorejection issues or moral concerns, and there is a need for a cell culture vessel and device that can effectively support various cell types.

Innovation Solution

A cell culture vessel with a bottom surface width equal to or greater than its side surface height, featuring a flow path for fluid supply and a closed interior, optionally with transparent surfaces, and connected to a variable volume container, allowing for fluid movement and substance interaction.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If a closed cell culture vessel with flow path is used, then cell culture efficiency and sterility are improved, but device complexity increases

Engineering Contradiction:

Improvecell culture efficiencyVSAvoiddevice complexity

Core Design Contradiction:

ProductivityVSDevice complexity

Solution Approach 1:

The cell culture vessel is divided into separate functional modules: a culture chamber for cell cultivation, a flow path system for fluid delivery, and a closed interface for sterilization. This segmentation allows each component to be optimized independently while maintaining overall system functionality, resolving the contradiction between improved culture efficiency and reduced device complexity.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

A flow path acts as an intermediary element connecting the external fluid supply system to the closed culture chamber. This intermediary enables nutrient and waste exchange without compromising the closed sterile environment, thereby improving cell culture efficiency while maintaining a relatively simple device structure.

Inventive Principle:

Principle #24Intermediary (Mediator)

2Difficulty of detecting and measuring

If transparent surfaces are used in the cell culture vessel, then observation capability is improved, but manufacturing complexity increases

Engineering Contradiction:

Improveobservation capabilityVSAvoidmanufacturing complexity

Core Design Contradiction:

Difficulty of detecting and measuringVSEase of manufacture

Solution Approach 1:

Transparent materials are applied locally only to the surfaces requiring observation (such as the culture chamber walls), while other portions of the device can use simpler, more cost-effective materials. This local application of transparency maintains excellent observation capability while minimizing manufacturing complexity and cost.

Inventive Principle:

Principle #3Local quality

3Adaptability or versatility

If the bottom surface width is equal to or larger than side surface height, then cell culture versatility is improved, but device complexity increases

Engineering Contradiction:

Improvecell culture versatilityVSAvoiddevice complexity

Core Design Contradiction:

Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The culture vessel is designed with a bottom surface width equal to or larger than side surface height, creating a versatile geometry that can accommodate various cell types and culture methods (adherent, suspension, 3D cultures). This universal design allows a single device structure to serve multiple culture purposes without increasing complexity.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Data Source

PatentUS20250250525A1Cell culture vessel and cell culture device

Publication Date: 2025.08.07 I PEACE INC

AI summary

A cell culture vessel for culturing a cell in an interior thereof, has a feature that a width of a bottom surface is equal to or larger than a height of a side surface. The cell culture vessel includes a flow path for supplying a fluid into the interior, and the interior is able to be closed.