Cell Culturing Device Discharge Pipe Positioning
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Solution Overview
Problem
The existing cell culturing device inaccurately aspirates and dispenses samples due to variations in gas concentration caused by oxygen bubbling during oxygen supply.
Innovation Solution
A cell culturing device with a vessel, agitator, discharge pipe, and oxygen intake pipe is designed, where the discharge pipe is positioned below the agitator in a vertical direction to minimize the impact of oxygen bubbling, ensuring a constant gas concentration in the culture solution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If oxygen is supplied to the culture solution, then the oxygen concentration is improved, but gas bubbles are generated causing inaccurate aspiration and dispensing
Solution Approach 1:
The harmful gas bubbles are separated from the culture solution by extracting them through the discharge pipe positioned at the bottom of the vessel. The discharge pipe extracts gas bubbles that accumulate at the bottom, preventing them from interfering with aspiration and dispensing operations while maintaining oxygen supply to the culture solution.
Solution Approach 2:
The discharge pipe is positioned in the vertical dimension at the bottom of the vessel, creating a separate gas removal pathway that operates independently from the horizontal aspiration and dispensing operations. This spatial separation in the vertical dimension allows simultaneous oxygen supply and accurate sampling without gas bubble interference.
2Use of energy by moving object
If oxygen bubbling is increased, then oxygen transfer efficiency is improved, but the proportion of gas in the culture solution varies
Solution Approach 1:
The system continuously removes gas bubbles through the discharge pipe positioned at the bottom, creating a feedback mechanism that maintains stable gas proportion in the culture solution. As gas bubbles accumulate from oxygen bubbling, they are automatically extracted through the discharge pipe, preventing composition instability while allowing efficient oxygen transfer.
Solution Approach 2:
Gas bubbles that accumulate as a byproduct of oxygen supply are continuously discarded through the discharge pipe. This discarding mechanism maintains stable gas proportion in the culture solution while allowing high oxygen transfer efficiency through active bubbling, as the harmful gas accumulation is continuously removed.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This configuration allows for accurate aspiration and dispensing of samples while maintaining a consistent amount of gas dissolved in the culture solution, improving the precision and reliability of the cell culturing process.
Implementation Method 1
an agitator that is provided in the vessel and agitates the culture solution
Implementation Method 2
an effect of oxygen bubbling caused by supplying oxygen
Data Source
AI summary
The cell culturing device includes a vessel that accommodates a culture solution containing a medium and cells, an agitator that is provided in the vessel and agitates the culture solution, a discharge pipe that discharges the culture solution in the vessel to outside the vessel, and an oxygen intake pipe that draws oxygen into the vessel. The discharge pipe has an end below the agitator in the vertical direction.