Tabletop Cell Delivery System with Constriction Cartridge
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Solution Overview
Problem
Existing methods for intracellular payload delivery are cumbersome, requiring multiple pieces of equipment and operators, which leads to inefficiencies, inconsistencies, and space constraints in laboratory settings.
Innovation Solution
A tabletop system designed for single-operator use, capable of processing cell suspensions through a disposable constriction cartridge to cause membrane perturbations, allowing payload delivery while controlling flow, temperature, agitation, and pressure.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If multiple separate pieces of equipment are used for cell preparation, payload delivery, and processing, then the delivery function can be achieved, but the device complexity increases and requires multiple operators
Solution Approach 1:
The patent combines multiple separate pieces of equipment (cell preparation device, payload delivery device, and processing equipment) into a single integrated tabletop system. The system includes a holder for cell suspension, a receiver for payload, a constriction cartridge for delivery, and control modules all integrated into one unit that can be operated by a single technician, thereby reducing operational complexity while maintaining delivery functionality
Solution Approach 2:
The tabletop system is designed to perform multiple functions within a single device: it can prepare cells, deliver payloads through constriction, and process cells after delivery. The system accepts different cell types and payload types, providing universal functionality that replaces multiple specialized devices while being controlled through a single interface
2Productivity
If multiple operators perform spatially and temporally distributed tasks, then specialized functions can be optimized, but the processing time increases due to coordination requirements
Solution Approach 1:
The system allows cell suspension to be prepared and loaded into the holder in advance, and the payload to be pre-loaded into the receiver. The constriction cartridge is pre-assembled with the delivery mechanism. These preliminary preparations eliminate the need for operators to perform tasks sequentially during the actual delivery process, thereby reducing coordination time and improving overall processing speed
Solution Approach 2:
The integrated system enables continuous operation where cell preparation, payload delivery, and post-delivery processing can occur in a continuous workflow without interruption for operator coordination. The automated control modules maintain continuous monitoring and adjustment of delivery parameters, ensuring uninterrupted processing that improves throughput compared to manual handoff between operators
3Area of stationary object
If numerous separate pieces of equipment are deployed, then functional capabilities are comprehensive, but the space occupation in laboratory clean-rooms increases
Solution Approach 1:
The system employs a nested structure where the constriction cartridge is inserted into the receiver, which is held by the holder, all within the compact tabletop housing. The control modules are integrated into the same housing. This nested arrangement allows multiple functional components to be space-efficiently arranged within a small footprint, fitting entirely on a laboratory benchtop while maintaining comprehensive delivery functionality
Solution Approach 2:
The system transitions from a horizontal arrangement of multiple separate equipment pieces occupying extensive bench space to a vertical, multi-layered integrated structure. The nested components are arranged in three-dimensional space within a compact housing, utilizing vertical dimensionality to pack multiple functions into a small footprint that occupies minimal laboratory clean-room space
4Reliability
If different operators operate different equipment, then specialized expertise can be utilized, but the consistency of results decreases across operators
Solution Approach 1:
The system incorporates control modules that continuously monitor delivery parameters such as constriction force, flow rate, and temperature. These sensors provide real-time feedback to the control system, which automatically adjusts parameters to maintain optimal delivery conditions. This closed-loop feedback ensures consistent results regardless of which operator is controlling the system, as the automated control compensates for manual variations
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Improves processing time, throughput rate, consistency, and efficiency of intracellular payload delivery, reducing the need for multiple operators and equipment, and optimizing space usage in clinical settings.
Implementation Method 1
the constriction cartridge configured to house a component comprising one or more cell-deforming constrictions, wherein the one or more cell-deforming constrictions are configured to cause perturbations in a cell membrane of the cell
Implementation Method 2
a flow control module configured to cause the cell suspension to flow from the input container through the disposable assembly to a cell suspension output container
Implementation Method 3
a pressure control module configured to cause pressure to be applied to the cell suspension
Implementation Method 4
a temperature control module configured to control a temperature of the cell suspension
Implementation Method 5
one or more control modules configured to control one or more of pressure, temperature, agitation, and flow of the cell suspension
Data Source
AI summary
A system for delivering a payload to a cell that includes: a platform supporting an input container, an output container, and a receiver for receiving all or part of a disposable assembly, the disposable assembly including a preparation vessel and a constriction cartridge. The preparation vessel holds a cell suspension as it is prepared for passage through one or more cell-deforming constrictions, and the constriction cartridge houses a component that includes the one or more cell deforming constrictions. Passage through the cell-deforming constrictions causes perturbations in cell membranes to allow entry of a payload into the cells. The system includes one or more processors configured to receive input from a user and to automatically control pressure, temperature, agitation, and/or flow of the cell suspension as it passes through the input container, through the preparation vessel, through the constriction cartridge, and to the output container.