Cell Delivery Syringe Assembly for High-Density Suspension Flow
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Solution Overview
Problem
Delivering high-density cell suspensions, such as those containing mesenchymal stem cells transiently-transfected by a polynucleotide encoding a Notch intracellular domain, is challenging due to needle blockage and cell damage when using fine needles, leading to inconsistent treatment results.
Innovation Solution
A method involving a syringe preparation process that includes aligning the barrel horizontally, using a micropipette to fill the lumen with the cell suspension, and employing a chemically inert plunger tip to minimize cell damage, followed by controlled injection through a cannula or needle, with rotational and retraction steps to ensure uniform delivery.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If a fine needle is used to deliver high-density cell suspensions, then precision of delivery is improved, but needle blockage occurs leading to cell damage
Solution Approach 1:
The system divides the delivery function into two separate components: a large-bore needle for reliable cell suspension delivery without blockage, and a separate fine-gauge cannula for precise target site delivery. This segmentation allows each component to optimize its specific function without compromise.
Solution Approach 2:
A hub assembly acts as an intermediary component that connects the large-bore needle and fine-gauge cannula, enabling the transfer of cell suspension from the needle to the cannula. This intermediary allows the system to benefit from both the reliability of the large needle and the precision of the fine cannula.
2Reliability
If a large-bore needle is used to deliver high-density cell suspensions, then needle blockage is prevented, but damage to the target site increases
Solution Approach 1:
The delivery system is segmented into two functional parts: a large-bore needle for reliable suspension delivery without blockage, and a separate fine-gauge cannula for precise, minimally invasive target site delivery. This allows the system to achieve both reliability and minimal tissue damage.
Solution Approach 2:
The hub assembly serves as an intermediary that transfers the cell suspension from the large-bore needle to the fine-gauge cannula, enabling the system to combine the advantages of both needle types while eliminating their respective disadvantages.
3Quantity of substance
If high concentration of cells is administered, then treatment effectiveness is improved, but needle blockage results in inconsistent treatment
Solution Approach 1:
The system segments the delivery function to allow high-concentration cell suspension to be delivered through a large-bore needle without blockage, then transferred to a fine-gauge cannula for precise administration. This ensures both high cell concentration and consistent delivery.
Solution Approach 2:
The hub assembly acts as an intermediary transfer mechanism that enables reliable passage of high-concentration cell suspension from the large-bore needle to the fine-gauge cannula, maintaining treatment consistency while achieving therapeutic cell concentrations.
Data Source
AI summary
A method of preparing a syringe in connection with a therapeutic treatment is disclosed. The method can include removing a plunger of the syringe from a barrel of the syringe, aligning the barrel in a horizontal orientation, filling a lumen of the barrel with a viscous material through an opening at a proximal end of the barrel, and inserting a plunger tip into the lumen to seal the lumen. The method can also include attaching an implantation device to a hub coupled to the barrel at a distal end of the barrel. The method can also include depressing the plunger until the cell suspension fills the implantation device and a droplet of the cell suspension is expelled from a distal tip of the implantation device.