Cell Destruction Verification via Indicator Assay
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Solution Overview
Problem
Conventional methods for verifying the destruction of cells lack effectiveness, as they do not provide strong verification that cells have been actually destroyed or mixed with a killing agent, and can be easily falsified by using vials without cells.
Innovation Solution
A method and system that generate a value of an indicator, such as a barcode or QR code, after running a medium containing dead cells through an assay, which can be used to verify the destruction of cells by comparing it to a reference value, ensuring the cells were properly destroyed and the agent used.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If conventional photo verification method is used, then ease of operation is improved, but reliability of verification deteriorates
Solution Approach 1:
An indicator substance is introduced as an intermediary between the cell destruction process and verification. The indicator is incorporated into the cell sample and produces a detectable signal only after destruction, serving as a mediator that proves destruction occurred without requiring direct observation of the destruction event itself.
Solution Approach 2:
The verification system utilizes color changes or other detectable signal changes of the indicator substance to verify cell destruction. The indicator transitions from an undetectable or different state before destruction to a detectable signal state after destruction, providing visual or measurable confirmation of the destruction event.
2Device complexity
If photo verification method is used, then device complexity is reduced, but measurement precision deteriorates
Solution Approach 1:
The mechanical/visual photo verification system is replaced with a chemical/biochemical detection system. Instead of using cameras and visual inspection, the system uses indicator substances that produce detectable signals (color changes, fluorescence, etc.) when cells are destroyed, substituting physical observation with chemical detection.
Solution Approach 2:
The indicator substance acts as a mediator that converts the biological event of cell destruction into a detectable signal. This intermediary enables precise measurement of destruction without requiring complex imaging or observation systems.
3Reliability
If indicator-based verification is implemented, then reliability of verification is improved, but device complexity increases
Solution Approach 1:
The cell destruction verification system performs self-verification through the indicator substance that is already incorporated into the cell sample. The destroyed cells themselves produce the verification signal through the indicator, eliminating the need for separate verification equipment or external validation systems.
Solution Approach 2:
The system uses simple color changes or detectable signal changes of the indicator substance to provide verification, avoiding the need for complex analytical instruments. The indicator's physical or chemical property change serves as the verification mechanism.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach provides a secure and reliable verification process that ensures the destruction of cells, preventing misuse and ensuring compliance with license agreements, by generating a unique indicator value that is unknown to the party destroying the cells until the assay is run.
Implementation Method 1
running a medium including a dead cell through an assay using a system. Running the medium through the assay may cause generation of a value of an indicator to signify that the assay has been run
Data Source
AI summary
Embodiments of the present invention allow for verifying the destruction of cells by another party. The destruction of cells can be verified by generating a value of an indicator (e.g., a bar code), where the value depends on some feature of the destruction. The party destroying the cells provides the value of the indicator as evidence that the cells have been destroyed. Embodiments may include a method of verifying the destruction of a cell. The method may include running a medium including a dead cell through an assay using a system. Running the medium through the assay may generate a value of an indicator to signify that the assay has been run. The value may identify the system, the cell, or the destruction mode of the cell. The method may further include receiving a verification code to acknowledge that the value is valid.