Cell Detachment Device with Closed-Loop Amplitude Feedback
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Solution Overview
Problem
Existing cell detachment methods using vibration energy face challenges in maintaining a stable amplitude, which affects the balance between cell detachment rate and survival rate, due to individual differences in culture substrates and their states.
Innovation Solution
A cell detachment device and method that include a vibration member, a vibration transmission member, a drive voltage output unit, an amplitude detection unit, and a drive voltage control unit, which together allow for the application of vibration at a stable amplitude by detecting and controlling the vibration amplitude to match the natural vibration frequency of the substrate, ensuring efficient cell detachment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If vibration energy is applied to detach cells from the substrate, then cell detachment is achieved, but the amplitude of vibration becomes unstable due to individual differences in substrates
Solution Approach 1:
The patent implements a feedback control system where a sensor detects the actual vibration amplitude of the substrate, and this detected value is fed back to a control unit that adjusts the drive voltage to the vibration generator. This closed-loop feedback mechanism ensures stable vibration amplitude despite variations in substrate properties, directly resolving the contradiction between detachment effectiveness and amplitude stability.
Solution Approach 2:
The system dynamically changes the drive voltage parameter based on detected substrate characteristics and real-time amplitude feedback. By adjusting the voltage parameter in response to substrate individual differences, the system maintains optimal vibration amplitude for cell detachment while ensuring stability across various substrate types.
2Productivity
If the drive voltage is increased to improve cell detachment, then detachment rate increases, but cell survival rate decreases due to excessive vibration
Solution Approach 1:
The feedback control system detects the actual vibration amplitude and adjusts the drive voltage accordingly, preventing excessive voltage application. This ensures cell detachment is achieved at optimal amplitude levels without causing damage from over-vibration, thus resolving the contradiction between detachment efficiency and cell survival.
Solution Approach 2:
The system applies vibration at the minimum necessary amplitude to achieve cell detachment, avoiding excessive action. By using feedback to determine the precise voltage needed for each substrate, the system applies just enough vibration for effective detachment while minimizing harmful effects on cell survival.
3Ease of operation
If a fixed drive voltage is used for vibration, then the device operation is simple, but the vibration amplitude varies due to substrate individual differences
Solution Approach 1:
The system performs self-adjustment by automatically detecting substrate characteristics and adjusting the drive voltage without user intervention. The feedback control mechanism enables the device to adapt to individual substrate differences autonomously, maintaining amplitude consistency while keeping the operation interface simple and unchanged.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables effective cell detachment with a stable amplitude, maintaining a balance between detachment rate and survival rate by adjusting the drive voltage based on detected amplitude, thus addressing the instability issues in previous methods.
Implementation Method 1
a vibration member (103) configured to be vibrated by a drive voltage at an amplitude depending on an output value of the drive voltage; a vibration transmission member (104) configured to vibrate by receiving vibration of the vibration member (103), and transmit the vibration of the vibration member (103) to a substrate (207)
Implementation Method 2
an amplitude detection unit (107) configured to detect an amplitude of vibration of the vibration transmission member (104)
Data Source
AI summary
Provided is a cell detachment device for detaching a cell on a substrate from the substrate by applying vibration, the cell detachment device including: a vibration member configured to be vibrated by a drive voltage at an amplitude depending on an output value of the drive voltage; a vibration transmission member configured to vibrate by receiving vibration of the vibration member, and transmit the vibration of the vibration member to the substrate; a drive voltage output unit configured to output the drive voltage; an amplitude detection unit configured to detect an amplitude of vibration of the vibration transmission member; and a drive voltage control unit configured to control the output value of the drive voltage, based on the amplitude detected by the amplitude detection unit. A cell detachment method which uses the cell detachment device is also provided.