Cell Detachment Using Curved Containers for Air-Free Vibration
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Solution Overview
Problem
Existing cell detachment methods face inefficiencies due to air entering the gap between the culture container and the acoustic transmission medium, leading to reduced vibration transmission and decreased cell detachment efficiency, which can also affect cell survival rates.
Innovation Solution
A cell detachment apparatus that stacks a vibrator, vibrating plate, and acoustic transmission medium in a specific configuration to excite bending vibrations on the culture container, ensuring contact at antinodes and avoiding nodes, thereby enhancing vibration transmission and minimizing air entry.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If deaeration processing is performed to remove air from the gap between the acoustic transmission medium and culture container, then air interference is eliminated, but cell survival rate decreases
Solution Approach 1:
The culture container bottom surface is designed with a predetermined curvature (concave or convex) before the cell detachment process. This preliminary structural design ensures that when the container is placed on the acoustic transmission medium, air is naturally excluded from the contact interface without requiring deaeration processing, thus maintaining both vibration transmission efficiency and cell survival rate
Solution Approach 2:
The invention applies curvature to the bottom surface of the culture container, transforming it from a flat surface to a curved surface. This curvature design ensures optimal contact with the acoustic transmission medium while preventing air entrapment, thereby eliminating the need for deaeration and avoiding harm to cultured cells
2Productivity
If vibration is applied to detach cells, then cell detachment efficiency improves, but air entry between acoustic transmission medium and culture container reduces vibration transmission
Solution Approach 1:
The culture container bottom surface is designed with a predetermined curvature before the cell detachment process. This preliminary design ensures that when the container is placed on the acoustic transmission medium, air is naturally excluded from the contact interface, enabling efficient vibration transmission without energy loss
Solution Approach 2:
The curved bottom surface of the culture container ensures optimal contact with the acoustic transmission medium during vibration application. This curvature design eliminates air gaps that would otherwise cause vibration reflection and energy loss, thereby improving vibration transmission efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This configuration improves cell detachment efficiency and survival rates by effectively transmitting vibrations to the culture container, reducing air interference, and allowing for high-efficiency cell collection without the need for proteolytic enzymes.
Implementation Method 1
a cell detachment apparatus that detaches cells placed on a culture surface of a culture container for cells by applying vibration to the cells
Implementation Method 2
a bending vibration is excited on the culture container when a vibration of the vibrating plate is transmitted to the culture container via the acoustic transmission medium
Implementation Method 3
the vibration of the vibrating plate is transmitted to the culture container via the acoustic transmission medium
Data Source
AI summary
A cell detachment apparatus that detaches cells placed on a culture surface of a culture container for cells by applying vibration to the cells includes a vibrator and a vibrating plate, wherein the vibrator, the vibrating plate, an acoustic transmission medium, and the culture container are stacked in this order, wherein a bending vibration is excited on the culture container when a vibration of the vibrating plate is transmitted to the culture container via the acoustic transmission medium, and wherein the acoustic transmission medium and the culture container come into contact with each other at a region including an antinode of the bending vibration and do not come into contact at a node of the bending vibration.