Cell Detachment Using Cryopreservation Liquid for Direct Freezing
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Solution Overview
Problem
Existing cell detachment methods, particularly the filter liquid replacement method, result in significant cell loss during the process of preserving detached cells, as many cells suspended in the detachment liquid are not recovered.
Innovation Solution
A cell detachment method involving the discharge of a cryopreservation liquid onto the culture surface to detach cells, followed by recovery of the detached cells with the same liquid, eliminating the need for a separate liquid replacement step and directly freezing the cell-suspended preservation liquid.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If a filter liquid replacement method is used to preserve detached cells, then cells can be recovered, but significant cell loss occurs during the process
Solution Approach 1:
The patent merges the detachment liquid and cryopreservation liquid into a single integrated liquid system. The detachment liquid is directly replaced with cryopreservation liquid without using a filter, combining the detachment function and preservation function into one continuous process, thereby preventing cell loss that occurs during separate filtration and replacement steps
Solution Approach 2:
The patent extracts and eliminates the filter component from the traditional liquid replacement process. By removing the filter step, the patent avoids the cell loss associated with filtration while maintaining the ability to recover and preserve detached cells effectively
2Ease of manufacture
If traditional filter liquid replacement method is used, then liquid replacement can be performed, but the process requires multiple steps increasing time and labor
Solution Approach 1:
The patent combines multiple liquid replacement steps into a single integrated operation. The detachment liquid is directly exchanged with cryopreservation liquid without intermediate filtration steps, reducing the number of operations required and thereby decreasing the total time and labor involved in the preservation process
3Quantity of substance
If filter liquid replacement method is used, then cells can be separated, but many suspended cells cannot be recovered
Solution Approach 1:
The patent removes the filter from the cell recovery process, extracting the harmful element that causes cell loss. By directly transferring the cell-suspended detachment liquid to the cryopreservation liquid without filtration, the patent ensures reliable recovery of all suspended cells including those that would be lost during filtration
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Reduces cell loss and minimizes damage by allowing direct freezing of the recovered cells, enabling efficient preservation with reduced labor and time compared to traditional methods.
Implementation Method 1
a liquid used for cryopreservation of cells is discharged toward a surface of a culture container in contact with the cells
Implementation Method 2
the detached cells are recovered together with the discharged liquid used for cryopreservation
Implementation Method 3
the cryopreservation liquid in which the cells are suspended is frozen and preserved
Data Source
AI summary
According to one embodiment, a cell detachment method comprising: a detachment step of discharging a liquid used for cryopreservation of cells toward a surface of a culture container in contact with the cells, where the cells are cultured, thereby detaching the cells from the surface; and a recovery step of recovering the detached cells together with the discharged liquid used for cryopreservation.