Cell Detachment Vibration Path Design for Low-Damage Collection

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Solution Overview

Problem

Existing cell detachment methods face inefficiencies due to complex vibration transmission paths and interference, leading to inconsistent cell detachment efficiency and potential cell damage.

Innovation Solution

A cell detachment apparatus and method that utilizes a vibrator, vibrating plate, and acoustic transmission medium, arranged to avoid contact with protruding portions of the culture container, ensuring a specific gravity between the protruding portion and vibrating plate is greater than 0 and less than 0.90, facilitating controlled vibration transmission.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If a vibrating device is used to detach cells from the culture container, then cell detachment efficiency is improved, but vibration transmission paths become complex and interfere with each other, reducing detachment efficiency

Engineering Contradiction:

Improvecell detachment efficiencyVSAvoidvibration transmission path complexity

Core Design Contradiction:

ProductivityVSDevice complexity

Solution Approach 1:

The invention extracts the protruding portion from the vibration transmission path by positioning the vibrating plate above it, eliminating the complex vibration paths that would otherwise occur through the protruding portion and its supporting legs

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The invention introduces an acoustic transmission medium as an intermediary between the vibrating plate and the culture container, which provides controlled vibration transmission while avoiding direct contact with the protruding portion

Inventive Principle:

Principle #24Intermediary (Mediator)

2Productivity

If vibrations are applied to detach cells, then cell detachment is achieved, but cell damage may occur due to uncontrolled vibration strength

Engineering Contradiction:

Improvecell detachment efficiencyVSAvoidcell damage

Core Design Contradiction:

ProductivityVSObject-affected harmful factors

Solution Approach 1:

The invention controls vibration parameters by adjusting the specific gravity of the acoustic transmission medium (0 < SG < 0.90) and the depth of the groove, thereby optimizing vibration strength to achieve detachment while minimizing cell damage

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The acoustic transmission medium acts as a mediator that transmits vibrations controllably to the culture container, preventing excessive vibration strength that would cause cell damage while maintaining sufficient detachment efficiency

Inventive Principle:

Principle #24Intermediary (Mediator)

3Force

If the vibrating plate contacts the protruding portion, then vibration transmission is enhanced, but multiple vibration paths create interference and complicate vibration control

Engineering Contradiction:

Improvevibration transmission forceVSAvoidvibration control difficulty

Core Design Contradiction:

ForceVSEase of operation

Solution Approach 1:

The invention removes the protruding portion from the vibration transmission path by positioning the vibrating plate above it, eliminating the multiple vibration paths that would create interference and complicate control

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The invention positions the vibrating plate in a different spatial dimension (above the protruding portion) rather than allowing contact with it, thereby avoiding the complex vibration paths while maintaining effective vibration transmission through the acoustic medium

Inventive Principle:

Principle #17Another dimension (Dimensionality change)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enhances cell detachment efficiency while minimizing cell damage by optimizing vibration transmission, allowing for high-yield cell collection without the need for proteolytic enzymes.

Implementation Method 1

an acoustic transmission medium, wherein the cell detachment apparatus is configured so that in a case where the vibrator, the vibrating plate, the acoustic transmission medium, and the culture container are arranged to be stacked in this order, the acoustic transmission medium and the vibrating plate do not come into contact with the protruding portion

Methodology Applied

Scientific EffectAcoustic transmission: Sound

Implementation Method 2

a vibrator, and a vibrating plate, wherein the culture container includes a protruding portion on a surface on an opposite side of the culture surface

Methodology Applied

Scientific EffectVibration: Vibration

Implementation Method 3

applying a vibration to the cells by applying a vibration from a vibrator to cells through a vibrating plate and an acoustic transmission medium

Methodology Applied

Scientific EffectUltrasonic vibration: Ultrasonic Vibration

Data Source

PatentUS20250257305A1Cell detachment apparatus and cell detachment method

Publication Date: 2025.08.14 CANON KK

AI summary

A cell detachment apparatus that detaches cells placed on a culture surface of a culture container for cells by applying a vibration to the cells, includes a vibrator, and a vibrating plate, wherein the culture container includes a protruding portion on a surface on an opposite side of the culture surface, and wherein the cell detachment apparatus is configured so that in a case where the vibrator, the vibrating plate, an acoustic transmission medium, and the culture container are arranged to be stacked in this order, the acoustic transmission medium and the vibrating plate do not come into contact with the protruding portion.