Cell Detachment System Using Vibration-Based Condition Setting
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Solution Overview
Problem
Existing cell detachment methods, such as those described in International Publication No. WO2016/047368, require advanced examinations for optimal detachment conditions and are inefficient in determining suitable detachment conditions for different culture vessels, while support devices like International Publication No. WO2020/039683 are unsuitable for improving cell detachment efficiency.
Innovation Solution
An information processing device and system that acquires cultivation information about the culture vessel and sets recommended detachment conditions by associating vessel-specific information with detachment conditions, including vibration frequency and intensity, to optimize cell detachment while minimizing damage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If vibration-based cell detachment is performed without advance examination of optimal conditions, then detachment can be performed quickly, but cell damage increases and detachment efficiency decreases
Solution Approach 1:
The system performs preliminary actions by pre-examining and storing optimal detachment conditions for various culture vessel types in advance. When a detachment operation is needed, the system retrieves pre-determined conditions (vibration frequency, intensity, duration) from the storage unit based on the specific vessel type, eliminating the need for time-consuming real-time optimization while ensuring reliable detachment results.
2Productivity
If vibration frequency and intensity are increased to improve detachment rate, then cell detachment efficiency improves, but cell damage increases
Solution Approach 1:
The system optimizes detachment by precisely controlling vibration parameters (frequency, intensity, duration) based on the specific culture vessel type. The storage unit contains pre-determined parameter combinations that achieve effective detachment while minimizing cell damage. The system retrieves and applies these optimized parameters automatically, avoiding both insufficient detachment and excessive cell damage that would result from arbitrary parameter selection.
3Ease of operation
If generic detachment conditions are applied to different culture vessel types, then operation simplicity is maintained, but detachment effectiveness varies and requires re-examination for each vessel type
Solution Approach 1:
The system achieves universality by creating a comprehensive database of optimal detachment conditions for multiple culture vessel types (flasks, dishes, wells, bioreactors, etc.). The storage unit stores vessel type information and corresponding optimal vibration parameters. The system can automatically retrieve and apply appropriate conditions for any vessel type, making the detachment process both simple to operate and highly adaptable to different vessel configurations without requiring re-examination for each type.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system effectively sets appropriate detachment conditions for various culture vessels, enhancing the efficiency and reliability of cell detachment processes by considering the unique properties of each vessel, thereby improving cell survival rates and detachment ratios.
Implementation Method 1
a vibration application unit configured to apply vibration to the culture vessel
Data Source
AI summary
The information processing device includes: a cultivation information acquisition unit configured to acquire cultivation information of a cell; and a recommended condition setting unit configured to set a recommended condition for detaching the cell from a to be-processed vessel in which the cell is cultured, the cultivation information including information about the to-be-processed vessel, the recommended condition being a condition for detaching the cell by applying vibration to the to-be-processed vessel, the recommended condition setting unit being configured to set the recommended condition based on the cultivation information, with use of information concerning an association relationship between information about a cultivation condition and information about a detachment condition, the information about the cultivation condition including information about a culture vessel, the information about the detachment condition including information about a condition for applying vibration to the culture vessel.