Cell Detachment Vibration Transfer Without Protrusion Interference
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Solution Overview
Problem
Existing cell detachment methods face inefficiencies due to multiple vibration transmission paths in culture containers, leading to inconsistent vibration application that can either be too strong or too weak, affecting cell detachment efficiency.
Innovation Solution
A cell detachment apparatus is designed with a specific configuration where a vibrator, vibrating plate, and acoustic transmission medium are stacked without direct contact with the culture container's protruding portion, using a substance with a specific gravity between 0 and 0.90 to optimize vibration transmission.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Strength
If the culture container includes a protruding portion on the bottom surface, then the culture container provides structural support and stacking capability, but multiple vibration transmission paths are created causing vibration interference and reducing cell detachment efficiency
Solution Approach 1:
The invention extracts the harmful vibration transmission path by removing the protruding portion from the culture container bottom surface. By eliminating this structural feature, the patent prevents the creation of multiple vibration transmission paths that cause interference, thereby resolving the contradiction between structural support and cell detachment efficiency.
Solution Approach 2:
The invention introduces an acoustic transmission medium as an intermediary between the vibrating plate and the culture container. This mediator optimizes vibration transmission to the culture surface while preventing unwanted vibration paths through the container bottom, thus maintaining cell detachment efficiency without compromising structural integrity.
2Productivity
If vibrations are applied strongly to detach cells efficiently, then cell detachment efficiency improves, but cell damage increases
Solution Approach 1:
The invention applies vibration locally and selectively to the culture surface where cells are located, rather than transmitting vibration uniformly throughout the entire culture container. By eliminating the protruding portion, the patent ensures that vibration energy is concentrated on the intended target (culture surface) without creating secondary vibration paths that could cause excessive or uneven vibration, thus achieving high detachment efficiency while minimizing cell damage.
3Power
If the acoustic transmission medium contacts the protruding portion, then vibration transmission is enhanced, but vibration control becomes complicated due to multiple transmission paths
Solution Approach 1:
The invention removes the protruding portion that creates complex vibration transmission paths. By extracting this structural element, the patent simplifies the vibration control system while maintaining effective vibration transmission through the acoustic transmission medium to the culture surface.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This configuration enhances cell detachment efficiency by ensuring consistent and effective vibration application, reducing interference and maintaining cell integrity.
Implementation Method 1
applying a vibration to the cells... in a case where the vibrator, the vibrating plate, an acoustic transmission medium, and the culture container are arranged to be stacked in this order
Data Source
Figure 1A~1B
Figure 2A~2B
Figure 3A~3B
AI summary
A cell detachment apparatus that detaches cells placed on a culture surface of a culture container for cells by applying a vibration to the cells, includes a vibrator, and a vibrating plate, wherein the culture container includes a protruding portion on a surface on an opposite side of the culture surface, and wherein the cell detachment apparatus is configured so that in a case where the vibrator, the vibrating plate, an acoustic transmission medium, and the culture container are arranged to be stacked in this order, the acoustic transmission medium and the vibrating plate do not come into contact with the protruding portion.