Programmed Cell Freezing with Feedback-Controlled Tube Lowering
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Solution Overview
Problem
The inconsistency in cooling rate and pre-cooling temperature during programmed cell freezing using liquid nitrogen fumigation leads to varying cell preservation effects.
Innovation Solution
A method and device for programmed cell freezing that controls the lowering speed of the cell preservation tube in a heat preservation chamber containing liquid nitrogen, continuously monitoring and adjusting the temperature difference inside and outside the tube to maintain it within a preset threshold, ensuring consistent cooling and reaching a preset temperature, using temperature distribution functions and real-time temperature measurements.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If liquid nitrogen fumigation is used for cell freezing, then equipment simplicity is improved, but cooling rate consistency and temperature control precision deteriorate
Solution Approach 1:
The patent employs real-time temperature monitoring during the cell freezing process using temperature sensors that continuously measure the temperature of the cell suspension. This feedback mechanism allows the system to detect temperature changes and adjust the lowering speed of the cell preservation tube accordingly, ensuring consistent cooling rates. The control system processes temperature data and dynamically modifies the lowering speed to maintain optimal cooling conditions, thereby resolving the contradiction between equipment simplicity and cooling rate consistency.
Solution Approach 2:
The patent implements dynamic control of the cell preservation tube lowering speed based on real-time temperature measurements. Instead of using a fixed lowering speed, the system continuously adjusts the speed according to the actual temperature conditions of the cell suspension. This dynamic adjustment mechanism enables the system to maintain precise temperature control and consistent cooling rates while using simple liquid nitrogen fumigation equipment, thus resolving the technical contradiction.
2Ease of manufacture
If liquid nitrogen fumigation is used for cell freezing, then equipment simplicity is improved, but temperature control precision deteriorates
Solution Approach 1:
The system uses real-time temperature feedback from sensors monitoring the cell suspension temperature to control the lowering speed of the cell preservation tube. This feedback loop enables precise control of the pre-cooling temperature by adjusting the lowering speed based on actual temperature conditions, ensuring consistent pre-cooling temperature despite the simplicity of the liquid nitrogen fumigation equipment.
Solution Approach 2:
The patent replaces complex mechanical temperature control systems with a control method that uses real-time temperature measurement and dynamic adjustment of lowering speed. Instead of using complex heating or cooling mechanisms, the system achieves precise temperature control by controlling the speed at which the cell preservation tube is lowered into the liquid nitrogen, substituting mechanical complexity with a simpler speed-controlled approach.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Improves cell preservation efficacy by maintaining consistent cooling rates and temperatures, enhancing the freezing process and resulting in higher cell resuscitation rates compared to traditional methods.
Implementation Method 1
The cell preservation tube is controlled to lower in the heat preservation chamber in real time at a real-time speed of v=(k-b)/2ah
Implementation Method 2
acquiring temperatures T1 and T2 inside and outside the cell preservation tube at a same time and at a same height in real time
Implementation Method 3
acquiring a cooling rate k and a temperature distribution function T=a×h2+b×h+c of a heat preservation chamber containing liquid nitrogen
Implementation Method 4
Method, system and device for programmed cell freezing
Implementation Method 5
When the pre-cooling is completed, samples are immersed in liquid nitrogen to complete the freezing
Data Source
AI summary
A method, system and device for programmed cell freezing is disclosed. The method includes: S1, acquiring a cooling rate k and a temperature distribution function T=a×h2+b×h+c of a heat preservation chamber containing liquid nitrogen; S2, controlling a cell preservation tube to lower in the chamber at a speed of v=(k−b)/2ah, and acquiring temperatures T1 and T2 inside and outside the tube at a same time and at a same height in real time during the lowering of the tube; S3, determining whether a difference Δt between T1 and T2 exceeds a preset temperature difference threshold, if yes, proceeding to S4, and if no, proceeding to S2 until the tube reaches a position corresponding to a preset temperature T0; and S4, controlling the cell preservation tube to stop until the difference Δt between T1 and T2 is less than or equal to the temperature difference threshold, and continuing S2.