Cell Ghost Preparation for Lymphocyte Culture Safety
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Solution Overview
Problem
Current methods for preparing feeder cells or antigen-presenting cells for in vitro lymphocyte culture are inefficient, costly, and pose safety risks due to the use of gamma irradiation, UV irradiation, and chemical treatments, which can lead to crosslinking or destruction of nucleic acids and residual live cells, hindering large-scale production and clinical application.
Innovation Solution
A method for preparing cell ghosts by washing and lysing feeder cells to remove intracellular components, leaving intact cell membranes that express cytokines capable of promoting lymphocyte proliferation and activation, allowing for safer and more effective large-scale production of lymphocyte boosters.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If gamma ray irradiation, UV irradiation, or chemical treatments are used to deactivate feeder cells, then the proliferative activity of feeder cells is inhibited, but nucleic acids are crosslinked or destroyed and residual live cells may remain, creating safety risks
Solution Approach 1:
The patent extracts and removes the harmful intracellular components (nucleic acids, cytoplasm) from the feeder cells while retaining the functional cell membrane. This is achieved through hypotonic lysis which causes the cell membrane to burst and release intracellular contents, followed by washing to remove the released nucleic acids and cytoplasmic debris, leaving only the intact cell membrane that can still present antigens and provide feeding functions without the safety risks of crosslinked nucleic acids
Solution Approach 2:
The patent segments the feeder cell into two distinct components: the functional cell membrane (retained) and the intracellular components (removed). By separating these components through controlled lysis and washing, the invention keeps the beneficial cell membrane structure intact while eliminating the harmful nucleic acids and cytoplasmic contents that could pose safety risks
2Reliability
If genetically engineered recombinant cells are used as feeder cells, then the regulation of lymphocyte proliferation and differentiation is enhanced, but the complexity of cell preparation and cost increase
Solution Approach 1:
The patent creates a simplified copy of the feeder cell function by using the cell membrane alone, which retains the essential antigen-presenting and feeding capabilities. This membrane copy eliminates the need for complex genetically engineered recombinant cells while maintaining the regulatory function for lymphocyte proliferation and differentiation
Solution Approach 2:
The patent uses a simple, inexpensive cell membrane preparation that can be easily produced and discarded after use. This disposable membrane preparation replaces the need for maintaining and preparing complex genetically engineered feeder cell lines, reducing both cost and operational complexity
3Reliability
If physical or chemical methods are used to inactivate feeder cells, then the proliferative activity is controlled, but the equipment cost increases and operation becomes less safe
Solution Approach 1:
The patent replaces the mechanical and chemical inactivation systems (gamma ray irradiation equipment, UV irradiation equipment, chemical treatments) with a simple biological lysis process using hypotonic solution. This substitution eliminates the need for expensive and potentially unsafe equipment while achieving effective inactivation through controlled osmotic lysis
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The cell ghosts effectively stimulate lymphocyte amplification and activation, offering a safer and more cost-effective alternative to traditional methods by maintaining the feeding and antigen-presenting functions of feeder cells without residual nucleic acids or toxic components, enabling strong immunization function and efficient lymphocyte production.
Implementation Method 1
lysing the washed cells to obtain the cell ghost
Data Source
AI summary
Provided is a method for preparing and using cell ghosts with active factors as a synergist of a lymphocyte in vitro culture. The method for preparing cell ghosts comprises: washing a cell to obtain a washed cell; and cleaving the washed cell to obtain cell ghosts, wherein the cell has cytokines capable of promoting the proliferation and differentiation of lymphocytes on their surface.