Cell Growth Regulation Platform for Unculturable Microorganisms

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Solution Overview

Problem

Current methods struggle to accurately control and regulate eukaryotic and microbial cell growth in vitro, particularly for unculturable microorganisms, and to effectively select antimicrobial agents that address microbial diversity and interactions with environmental factors.

Innovation Solution

An automated system and method for cultivating unculturable bacteria and fungi, allowing for the growth of microorganisms with desired properties, and selecting effective antimicrobial agents by simulating their interaction with environmental factors and host factors, using a variety of media and supplements, and analyzing growth through imaging and genetic methods.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional culture methods are used, then common microorganisms can be grown, but previously unculturable microorganisms cannot be grown

Engineering Contradiction:

Improveability to cultivate microorganismsVSAvoidsuccess rate of culturing

Core Design Contradiction:

Adaptability or versatilityVSReliability

Solution Approach 1:

The patent applies parameter changes by systematically varying multiple culture conditions including oxygen concentration (aerobic/anaerobic), temperature ranges, pH levels, nutrient compositions, and incubation times to create optimized culture environments that enable growth of previously unculturable microorganisms

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The automated test system performs multiple functions: it cultivates diverse microorganisms under various conditions, tests antimicrobial susceptibility, identifies microbial species, and screens for novel compounds, replacing multiple separate laboratory procedures with a single integrated platform

Inventive Principle:

Principle #6Universality (Multi-functionality)

2Productivity

If manual methods are used for selecting antimicrobial agents, then basic testing can be performed, but rapid and accurate selection for diverse microbial communities is difficult

Engineering Contradiction:

Improvespeed of antimicrobial selectionVSAvoidaccuracy of antimicrobial efficacy assessment

Core Design Contradiction:

ProductivityVSMeasurement precision

Solution Approach 1:

The patent replaces manual mechanical procedures with automated systems including robotic liquid handling for precise reagent dispensing, automated incubation with controlled environmental parameters, and digital imaging systems for objective measurement of microbial growth and antimicrobial effects, eliminating human error and increasing throughput

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The system incorporates real-time feedback through automated monitoring of microbial growth parameters, with results fed back into the analysis algorithm to rapidly determine antimicrobial susceptibility patterns and guide selection of effective agents based on quantitative data rather than subjective assessment

Inventive Principle:

Principle #23Feedback

3Speed

If simple culture media are used, then growth of fast-growing bacteria is possible, but growth of slow-growing or fastidious microorganisms is inhibited

Engineering Contradiction:

Improvegrowth rate of microorganismsVSAvoidrange of cultivable microorganisms

Core Design Contradiction:

SpeedVSAdaptability or versatility

Solution Approach 1:

The patent employs dynamic culture conditions where parameters such as oxygen tension, nutrient supply, and environmental factors are adjusted over time to match the specific requirements of different microbial groups, allowing both fast-growing and slow-growing organisms to thrive in the same system under their respective optimal conditions

Inventive Principle:

Principle #15Dynamics

Data Source

PatentUS20260071248A1Products for regulation of eukaryotic and microbial cells growth

Publication Date: 2026.03.12 TETS VICTOR

AI summary

The invention relates to methods for the flexible regulation of cellular growth of eukaryotic and prokaryotic cells. In particular embodiments, regulation of eucaryotic and microbial cells growth, occurs by the control of their interaction with the environmental factors, nutrient media, media additives, supplements.