Cell Culture Imaging Calibration With Automated GUI Alignment
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Solution Overview
Problem
Cell culture imaging systems require precise calibration to ensure accurate measurements, but current methods are laborious and require highly trained personnel, making them inefficient and prone to errors.
Innovation Solution
An imaging system setup program with a graphical user interface (GUI) provides tools for aligning hardware components, capturing real-time or time-lapse video, and offering image processing and algorithms to enhance calibration and analysis, including digital holographic microscopy for non-invasive, marker-free analysis of cell cultures.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If manual calibration methods are used to ensure measurement accuracy, then measurement precision is improved, but device complexity and operation difficulty increase
Solution Approach 1:
The system performs self-calibration using automated image processing algorithms and machine learning models that analyze captured images and automatically adjust calibration parameters without requiring manual intervention or specialized training, thereby maintaining measurement accuracy while reducing operational complexity
Solution Approach 2:
Manual mechanical calibration procedures are replaced with computational image processing and automated algorithms that analyze image data to determine calibration parameters, substituting physical adjustment mechanisms with software-based solutions that reduce complexity while preserving precision
2Measurement precision
If manual calibration by highly trained personnel is used, then measurement accuracy is improved, but productivity decreases
Solution Approach 1:
The calibration system operates autonomously by capturing images, processing them through automated algorithms, and adjusting calibration parameters without human intervention, eliminating the time-consuming manual calibration process while maintaining accuracy standards
Solution Approach 2:
The system performs preliminary calibration actions automatically during system initialization or setup phases using pre-programmed algorithms and reference images, preparing the system for accurate measurements before actual operation begins, thereby eliminating the need for time-consuming manual calibration during use
3Measurement precision
If complex calibration procedures are used to ensure accuracy, then measurement precision is improved, but ease of operation deteriorates
Solution Approach 1:
The system automatically performs calibration through self-service mechanisms including automated image capture, processing, and parameter adjustment without requiring user expertise or complex manual procedures, making the system easy to operate while maintaining precision
Solution Approach 2:
The system uses reference images or standard samples as copies to establish calibration parameters, comparing captured images against known references to automatically determine calibration settings, thereby simplifying the operator's task while ensuring accuracy through standardized comparison
Data Source
AI summary
A method and apparatus for calibrating an imaging system provides a program stored in the imaging system having a graphical user interface and providing a plurality of tools to align hardware components of the imaging system including cameras for imaging cell culture vessels received by the imaging system and having a tool for providing a video image output of a cell culture vessel received in the imaging system.