Cell Isolation Apparatus with Dynamic Blade Control
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Solution Overview
Problem
Existing cell isolation techniques from body tissue are inefficient, requiring long operation times and resulting in low cell survival rates due to collisions with resistive elements and variability in mincing and enzymatic treatment processes.
Innovation Solution
A cell isolation apparatus with a chamber body, an isolation member with blade members that can be controlled by a CPU-driven system, including a liquid level sensor and temperature control, to optimize tissue dissociation and cell retrieval, ensuring high survival rates and efficient operation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If tissue is minced with a sharp blade and subjected to repeated enzymatic treatment with collagenase, then cells can be isolated from tissue, but the operation time period becomes very long and cell retrieving rate becomes dispersed
Solution Approach 1:
The invention extracts and removes the resistive element from the chamber body, eliminating the source of cell collision and damage. This allows cells to be retrieved without the harmful resistance that previously caused dispersed retrieving rates and required repeated treatment cycles, thereby reducing operation time while maintaining high cell retrieval rates
Solution Approach 2:
The invention introduces a controllable isolation member that can be dynamically adjusted in terms of rotation speed, rotation direction, and lifting/lowering movements. This dynamic control enables optimization of tissue dissociation efficiency, achieving high cell retrieval rates in shorter time by adapting the dissociation process to the specific tissue characteristics
2Quantity of substance
If cells are subjected to repeated enzymatic treatment and retrieval procedures, then more cells can be obtained, but cell survival rate is lowered due to collisions with resistive element
Solution Approach 1:
The resistive element is completely removed from the chamber body, eliminating the harmful collision mechanism that reduced cell survival rates. This allows multiple enzymatic treatment cycles to be performed without the damaging effect of cell-resistive element collisions, thereby maintaining high cell survival rates while achieving sufficient cell yields
Solution Approach 2:
The invention incorporates detection means to monitor the state of tissue dissociation and cell release in real-time. This feedback information is used by the control unit to adjust the isolation member's rotation speed and lifting/lowering movements, optimizing the dissociation process to maximize cell yield while preserving cell survival rates through precise control
3Productivity
If a resistive element is provided in the chamber to resist tissue movement during dissociation, then tissue dissociation effectiveness is improved, but cell survival rate is reduced due to cell collisions with the resistive element
Solution Approach 1:
The resistive element is extracted and removed from the chamber body, completely eliminating the source of cell collisions. The invention achieves tissue dissociation effectiveness through the isolation member's rotation and lifting/lowering movements alone, without requiring any resistive element, thereby maintaining high cell survival rates
Solution Approach 2:
The isolation member performs dynamic lifting and lowering movements in addition to rotation, creating effective tissue dissociation through the interaction between the moving isolation member and the tissue-enzyme solution mixture. This dynamic approach replaces the static resistive element mechanism, achieving effective dissociation without cell collisions
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The apparatus significantly enhances cell survival rates and reduces operation time by controlled tissue dissociation and enzyme activation, achieving higher cell yields with reduced cell damage.
Implementation Method 1
The shaft member may be rotated by a magnetic rotation driving portion which is not in contact with the isolation member.
Implementation Method 2
The cell isolation apparatus may further include a liquid level sensor which detects a level of a solution in the chamber.
Implementation Method 3
The cell isolation apparatus may further include a temperature controlling unit which controls one of a temperature in the chamber body and a temperature of the cell isolation apparatus.
Implementation Method 4
tissue is minced with a sharp blade, and the minced tissue is subjected to enzymatic treatment with collagenase or the like to digest the extracellular matrix, thereby obtaining isolated cells.
Data Source
AI summary
A cell isolation apparatus includes: a chamber body which includes a chamber into which tissue is to be introduced; an isolation member which is moved in the chamber and which is to collide with the tissue to isolate a cell; and a controlling portion which controls an operation of the isolation member in the chamber body.