Cell Isolation Device Blade Cutting Mechanism

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Solution Overview

Problem

Existing cell isolation processes in regenerative medicine are labor-intensive and inefficient, leading to insufficient manufacturing capacity and high cell damage rates, which hinder the demand for cell products.

Innovation Solution

A cell isolation method and device that utilize a mechanical operation to cut biological tissue into small pieces using a blade, with controlled pressing force and position change, followed by processing liquids to decompose and isolate cells, ensuring a high survival rate.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If manual cell isolation processing is performed by skilled workers, then cell damage can be controlled, but manufacturing capacity is insufficient

Engineering Contradiction:
Improvecell survival rateVSAvoidmanufacturing capacity
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent replaces manual mechanical operations with an automated mechanical cutting system consisting of a blade, blade receiver, and driving unit. This automation maintains controlled cutting forces while dramatically increasing processing capacity, resolving the contradiction between manual control quality and automated production volume.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent controls the pressing force parameter to be the minimum magnitude required for cutting, and optimizes cutting speed and position parameters. This parameter optimization ensures gentle tissue processing that protects cell viability while enabling automated high-volume processing.

Inventive Principle:
Principle #35Parameter changes

2Productivity

If automated cell isolation processing is implemented, then manufacturing capacity is improved, but cell damage increases

Engineering Contradiction:
Improvemanufacturing capacityVSAvoidcell survival rate
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent applies only the minimum necessary pressing force for cutting, avoiding excessive mechanical stress on cells. This partial action principle enables automated processing while maintaining cell integrity by applying just enough force to achieve cutting without causing damage.

Inventive Principle:
Principle #16Partial or excessive action

Solution Approach 2:

The automated mechanical system replaces manual operations with precisely controlled blade movements, consistent cutting forces, and standardized processing parameters, achieving both high throughput and cell protection through mechanical precision.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Ease of manufacture

If cutting processing is performed with high pressing force, then tissue is effectively cut, but cell damage increases

Engineering Contradiction:
Improvecutting efficiencyVSAvoidcell survival rate
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The patent applies the minimum necessary pressing force for effective cutting rather than high force. This approach achieves sufficient tissue division while minimizing mechanical stress on cells, maintaining both cutting efficiency and cell viability.

Inventive Principle:
Principle #16Partial or excessive action

Solution Approach 2:

The patent optimizes the pressing force parameter to a specific range that is sufficient for cutting but low enough to protect cells. This parameter optimization resolves the contradiction between cutting effectiveness and cell protection.

Inventive Principle:
Principle #35Parameter changes

4Device complexity

If blade cutting position is fixed, then processing is simple, but tissue division completeness decreases

Engineering Contradiction:
Improveprocessing complexityVSAvoidtissue division completeness
Core Design Contradiction:
Device complexityVSManufacturing precision

Solution Approach 1:

The patent employs a dynamic cutting system where the blade position changes during processing. The driving unit moves the blade to multiple positions on the blade receiver, enabling complete tissue division while maintaining relatively simple device architecture through programmed motion sequences.

Inventive Principle:
Principle #15Dynamics

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method and device effectively improve the manufacturing capacity of cell products by minimizing cell damage and ensuring a high survival rate, while also reducing the risk of contamination and promoting efficient decomposition and isolation processes.

Implementation Method 1

performing cutting processing of pressing a blade against a blade receiver and pressing and cutting the biological tissue disposed on the blade receiver, by a mechanical operation

Methodology Applied
Scientific EffectMechanical Force: Mechanical Force

Implementation Method 2

introducing a first processing liquid into the container to decompose the small pieces of the biological tissue in the container

Methodology Applied
Scientific EffectDecomposition (biological): Decomposition (biological)

Data Source

PatentEP4497817A1Cell isolation method and cell isolation apparatus
Publication Date: 2025.01.29 FUJIFILM CORP
  • EP4497817A1 patent drawingFigure 1
  • EP4497817A1 patent drawingFigure 2
  • EP4497817A1 patent drawingFigure 3A~3B

AI summary

A cell isolation method of isolating a cell from a biological tissue includes cutting processing of pressing a blade against a blade receiver and pressing and cutting the biological tissue disposed on the blade receiver.