Cell Lysis Composition Merging Nucleic Acid Extraction and PCR
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Solution Overview
Problem
Conventional molecular diagnostic methods require a separate nucleic acid extraction process, which is time-consuming and costly, especially in emergency situations and for rapid virus quarantine, due to the need for dedicated devices and consumables.
Innovation Solution
A molecular diagnostic method using a cell lysis composition containing Tween 20 and distilled water, which allows for simultaneous nucleic acid extraction and amplification during the polymerase chain reaction without a separate extraction process, by preparing a mixture with the cell lysis composition, a nucleic acid-containing sample, a premix, and a solution containing primers and a probe.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If a separate nucleic acid extraction process is performed using conventional methods, then high purity nucleic acid can be extracted, but the extraction process requires a long period (about 60 minutes) and dedicated extraction devices and consumables
Solution Approach 1:
The patent combines the nucleic acid extraction process with the PCR amplification process into a single unified reaction system. The cell lysis composition containing Tween 20 serves dual purposes: it extracts nucleic acids from cells and simultaneously provides the reaction environment for PCR amplification, eliminating the need for separate extraction and amplification steps
Solution Approach 2:
The cell lysis composition is designed to perform multiple functions: cell membrane disruption, nucleic acid release, and serving as a buffer for PCR reaction. This multi-functional composition eliminates the need for dedicated extraction devices and consumables, as the same reagents are used for both extraction and amplification
2Manufacturing precision
If conventional extraction methods are used, then nucleic acid can be extracted with high purity, but dedicated extraction devices and consumables must be continuously used, incurring high costs
Solution Approach 1:
The patent merges extraction reagents with PCR reagents into a single cell lysis composition, eliminating the need for separate extraction kits, magnetic beads, and dedicated extraction devices. This consolidation significantly reduces consumable costs and simplifies the diagnostic workflow
Solution Approach 2:
The cell lysis composition serves multiple purposes including cell lysis, nucleic acid extraction, and PCR reaction buffer, replacing multiple dedicated extraction consumables with a single multi-functional reagent system that reduces overall diagnostic costs
3Quantity of substance
If a separate nucleic acid extraction process is performed, then adequate nucleic acid can be obtained for PCR, but the overall molecular diagnosis time is extended
Solution Approach 1:
The patent combines extraction and amplification into a single simultaneous process where the cell lysis composition extracts nucleic acids while the PCR reaction amplifies them in the same reaction vessel and time frame, achieving both adequate nucleic acid quantity and rapid diagnosis
Solution Approach 2:
The cell lysis composition is pre-formulated to contain all necessary components for both extraction and PCR reaction, including buffers, enzymes, and primers, allowing the extraction and amplification to proceed simultaneously without sequential processing delays
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method reduces the time and cost of molecular diagnosis by eliminating the need for dedicated extraction devices and consumables, while achieving effective nucleic acid extraction and amplification.
Implementation Method 1
a cell lysis composition for nucleic acid extraction containing Tween 20 and distilled water
Data Source
Figure 1
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AI summary
The present invention relates to a molecular diagnostic method using a cell lysis composition for nucleic acid extraction, and particularly, to a molecular diagnostic method using a cell lysis composition for nucleic acid extraction, which may minimize the time for molecular diagnosis by performing a polymerase chain reaction on a mixture containing both Tween 20 as a component for nucleic acid extraction and a PCR buffer necessary for performing the polymerase chain reaction, without a separate nucleic acid extraction process, and reduce the cost of molecular diagnosis by minimizing the use of dedicated devices and consumables in extraction.