Cell Culture Medium With Ultra-Fine Bubbles for 3D Cell Oxygenation

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Existing methods struggle to efficiently produce large quantities of two-dimensional and three-dimensional cells due to oxygen supply issues leading to necrosis, hindering the production of spheroids and organoids in regenerative medicine.

Innovation Solution

A cell culture method utilizing a culture medium containing ultra-fine bubbles generated by film boiling on a heating element at the liquid interface, enhancing oxygen supply and cell growth.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If three-dimensional cell culture is performed to produce spheroids and organoids, then cell survival rate after transplantation is improved, but oxygen supply becomes insufficient leading to necrosis

Engineering Contradiction:

Improvecell survival rateVSAvoidoxygen supply insufficiency

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent changes the physical state of oxygen delivery by transforming dissolved oxygen into ultra-fine bubble form. This parameter change in oxygen delivery mechanism allows sufficient oxygen supply to three-dimensional cell structures without causing necrosis, while maintaining high cell survival rates after transplantation.

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent utilizes phase transition of oxygen from dissolved state to bubble phase by generating ultra-fine bubbles through film boiling. This phase transition enables enhanced oxygen supply to three-dimensional cells, preventing oxygen deprivation and necrosis while improving cell survival outcomes.

Inventive Principle:

Principle #36Phase transitions

2Ease of manufacture

If conventional culture methods are used, then production process is simple, but large-scale production of two-dimensional and three-dimensional cells is inefficient

Engineering Contradiction:

Improveproduction process simplicityVSAvoidcell production efficiency

Core Design Contradiction:

Ease of manufactureVSProductivity

Solution Approach 1:

The patent modifies the culture medium by incorporating ultra-fine bubbles, changing the oxygen delivery parameter. This enables efficient large-scale production of both two-dimensional and three-dimensional cells while maintaining operational simplicity through the use of a heating element for bubble generation.

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method enables efficient production of large amounts of two-dimensional and three-dimensional cells by preventing necrosis through improved oxygen supply, ensuring high cell survival rates.

Implementation Method 1

ultra-fine bubbles generated by heating a heating element to make film boiling on an interface between a liquid and the heating element

Methodology Applied

Scientific EffectFilm boiling: Boiling

Data Source

PatentUS12545872B2Cell culture method, method for producing culture medium, culture medium and culture device

Publication Date: 2026.02.10 CANON KK

AI summary

A large amount of three-dimensional cells are efficiently produced. To this end, a cell is cultured by using a culture medium containing an ultra-fine bubble generated by heating a heating element to make film boiling on an interface between a liquid W and the heating element.