Cell Observation Device Electrode Region Segmentation
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Solution Overview
Problem
Existing cell observation systems face challenges in achieving highly sensitive light analysis from samples due to non-reactive regions within the observation area and lack of defined observation regions in multiwell plates.
Innovation Solution
A cell observation system with electrode pairs placed within holding units in a sample case, where light detection and analysis units set specific regions facing the positive electrode as analysis areas, enhancing the ratio of optical intensity from cellular reactions to noise.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Area of stationary object
If the observation region is set as the circular region in the middle between electrodes, then the entire well area can be observed, but non-reactive regions are included reducing measurement sensitivity
Solution Approach 1:
The patent divides the observation region into multiple analysis regions based on electrode positions. Instead of treating the entire circular region as one analysis area, it segments it into multiple regions (e.g., first analysis region, second analysis region) corresponding to different electrode pairs, allowing selective analysis of reactive regions while excluding non-reactive areas.
Solution Approach 2:
The patent applies different analysis criteria to different regions within the observation area. By setting analysis regions specifically around electrode positions where cellular reactions occur, it ensures that only regions with relevant biological activity are analyzed, improving measurement sensitivity while maintaining adequate observation coverage.
2Ease of operation
If no specific observation region is defined, then the device can be simpler to operate, but the measurement sensitivity and precision are reduced
Solution Approach 1:
The patent pre-defines analysis regions based on electrode positions before actual measurement begins. The system automatically determines analysis regions using stored electrode position information, eliminating the need for manual region definition during operation while ensuring high measurement precision through predetermined optimal analysis areas.
Solution Approach 2:
The system automatically identifies and sets analysis regions based on electrode positions and cellular reaction patterns without requiring manual intervention. The device performs self-calibration and region definition, maintaining measurement sensitivity while simplifying operation through automated processes.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for highly sensitive analysis of light from samples by efficiently identifying and isolating regions reacting to electrical stimulation, thereby improving measurement sensitivity.
Implementation Method 1
electrode pairs including positive and negative electrodes are placed within a plurality of holding units arranged in a sample case, light from a sample within the holding units is detected by a light detection unit in a state where an electric field is generated by the electrode pairs
Implementation Method 2
a cell observation system and a cell observation method which measure light emitted from a sample including a cell when a voltage is applied thereto
Data Source
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AI summary
A cell observation system 1, for measuring fluorescence emitted from a cell held by a microplate 20 having a plurality of wells 21, comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, a position controller 30 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21, a moving image acquisition unit 40 for detecting the fluorescence from the sample S within the wells 21, and a data analyzer 50 for setting a part of a region facing the positive electrode 17b on the well 21 as an analysis region and analyzing an optical intensity in the analysis region so as to acquire analysis information concerning the sample S.