Cell Peeling Recognition via Inertial Cell Separation
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Solution Overview
Problem
Existing methods for recognizing the peeled state of cells in a culture vessel are inaccurate due to reliance on static images and stationary culture vessels, which fail to reliably distinguish between peeled and unpeeled cells.
Innovation Solution
A cell peeling recognizing device and method that involves moving the culture vessel to utilize the moment of inertia, allowing peeled cells to move within the imaging range, enabling multiple imaging captures and precise comparison of imaging data to determine the peeled state with high accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If static images are taken of cells in a stationary culture vessel, then the imaging process is simple and quick, but the recognition accuracy of peeled cells is insufficient
Solution Approach 1:
The patent transforms the stationary culture vessel into a dynamic system by moving it in the imaging direction. This allows peeled cells to separate from unpeeled cells through relative motion, enabling accurate recognition. The culture vessel is moved to cause peeled cells to move in the imaging direction while unpeeled cells remain relatively stationary, creating dynamic separation for better detection.
2Measurement precision
If the culture vessel is moved to utilize moment of inertia for cell separation, then the recognition precision improves, but the imaging process becomes more complex
Solution Approach 1:
The patent applies periodic vibration to the culture vessel in the imaging direction. This periodic action causes peeled cells to separate and move while unpeeled cells remain attached. The vibration is applied at appropriate frequencies and amplitudes to achieve effective cell separation without damaging the cells or requiring complex operational procedures.
3Measurement precision
If multiple imaging captures are performed to compare imaging data, then the peeled state can be accurately determined, but the imaging time increases
Solution Approach 1:
The patent performs preliminary movement or vibration of the culture vessel before imaging to separate peeled cells from unpeeled cells. This preliminary action ensures that when imaging is performed, the cells are already positioned for optimal detection. By preparing the sample beforehand, the actual imaging process requires fewer captures and less time while maintaining high accuracy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution allows for reliable recognition of peeled cells by imaging the culture vessel multiple times as cells move due to inertia, improving the precision of determining the peeled state and enabling accurate calculation of peeling rates.
Implementation Method 1
when the culture vessel is accelerated or decelerated its moving speed or stopped in the imaging range, cells that have been peeled from the culture vessel move inside the culture vessel due to the moment of inertia but cells that have not been peeled from the culture vessel cannot move inside the culture vessel
Data Source
AI summary
The present invention relates to a cell peeling recognizing device and a cell peeling recognizing method.When a moving part (robot 6) moves culture vessel 5, imaging device 14 images an interior of culture vessel 5 a plurality of times as the cells move inside the culture vessel due to the moment of inertia, and recognizing part 10a recognizes the peeled state of cells C by comparing various sets of the captured imaging data. The peeled state of cells in the culture vessel can be determined with high precision.