Cell Potential Measuring Device with Test Line Validation
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Solution Overview
Problem
Conventional multi-electrode arrays face challenges in determining whether variability in measurement results is due to the array or the cell itself, as issues with microelectrodes and measurement lines can lead to inconsistent signal acquisition.
Innovation Solution
A cell potential measuring device is designed with a base plate, insulation layer, measurement line, measurement electrode, and test line, where the test line is positioned below the measurement electrode through the insulation layer, allowing for the testing of the electrically conductive state of the measurement electrode and measurement line.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a conventional multi-electrode array is used for measuring cell action potentials, then measurement of cellular electrical activity is enabled, but it becomes impossible to determine whether variability in measurement results is caused by the array or the cell itself due to potential defects in microelectrodes and measurement lines
Solution Approach 1:
The measurement system is segmented into distinct functional components: measurement electrodes for signal acquisition, test electrodes for validation, measurement lines for data transmission, and test lines for system verification. This segmentation allows independent testing of each component to determine the source of measurement variability.
Solution Approach 2:
The test lines and test electrodes are pre-configured alongside the measurement system to enable preliminary validation of electrode and line functionality before actual cell measurements begin. This preliminary action ensures the measurement system is functioning correctly prior to data collection.
2Measurement precision
If reference electrodes are arranged as far as possible from microelectrodes to lower noise levels, then measurement noise is reduced, but the device complexity and space requirements increase
Solution Approach 1:
Test lines serve as intermediary pathways that allow validation of measurement system functionality without requiring additional reference electrodes positioned far from measurement electrodes. The test lines provide a controlled path for verifying electrode and line integrity within the existing measurement array footprint.
Data Source
AI summary
A cell potential measuring device includes a base plate having insulation properties, first line disposed on the base plate, an insulation layer disposed on the base plate and covering at least a surface of the first line, a first electrode disposed on the insulation layer and being electrically connected to the first line, and a second line disposed on the base plate and a portion of which is disposed below the first electrode via the insulation layer.