Cell Preservative Solution Stabilizing DNA
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Solution Overview
Problem
Current cell preservative solutions fail to maintain the stability and integrity of DNA and cells during storage, leading to degradation and inappropriate analysis results.
Innovation Solution
A cell preservative solution containing a lower alcohol with 1 to 6 carbon atoms and a divalent metal ion in an aqueous solvent, with a concentration of the divalent metal ion ranging from 6 mmol/L to 82 mmol/L, is used to effectively preserve and analyze cells by stabilizing DNA and maintaining cellular structure.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional cell preservative solutions are used, then cells can be stored before analysis, but DNA degradation occurs and cellular integrity is lost
Solution Approach 1:
The patent applies parameter changes by optimizing the concentration of divalent metal ions (specifically magnesium ions at 6-82 mmol/L) in the cell preservative solution. This specific concentration range stabilizes DNA during storage without causing degradation, resolving the contradiction between maintaining DNA stability and enabling long-term storage. The lower alcohol component (20-60% v/v) further contributes to this parameter optimization by preventing DNA degradation while maintaining cellular integrity throughout the storage period.
2Loss of time
If cells are stored for extended periods, then analysis can be performed later, but cellular structure degrades and analysis accuracy decreases
Solution Approach 1:
The patent uses an intermediary substance approach by incorporating divalent metal ions (magnesium ions) as a mediator between the cell structure and storage conditions. These ions act as a protective intermediary that maintains cellular structure integrity during extended storage periods, allowing time flexibility for analysis while preventing the degradation that would otherwise occur. The lower alcohol component serves as another intermediary that stabilizes cellular structures against the harmful effects of prolonged storage.
3Reliability
If cell preservative solution contains divalent metal ions, then DNA stability improves, but solution composition complexity increases
Solution Approach 1:
The patent applies local quality by focusing on the specific local concentration of divalent metal ions (6-82 mmol/L magnesium ions) within the preservative solution rather than uniformly distributing multiple different additives. This localized optimization of a single key component achieves DNA stability without the complexity of a multi-component formulation. The lower alcohol content (20-60% v/v) is similarly optimized at a specific concentration range to provide the necessary protective function without adding unnecessary compositional complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution allows for stable storage and analysis of cells, preventing DNA degradation and maintaining cellular integrity, even at varying temperatures, thereby ensuring accurate analytical results.
Implementation Method 1
A cell preservative solution containing a lower alcohol with 1 to 6 carbon atoms and a divalent metal ion in an aqueous solvent, wherein the concentration of the divalent metal ion is from about 6 mmol/L to about 82 mmol/L
Implementation Method 2
The present invention provides a method of preparing fixed cells, comprising contacting cells in vitro with the cell preservative solution so as to make the cells fixed
Data Source
AI summary
The present invention relates to a method of preserving cells in a cell preservative solution. The cell preservative solution comprises a lower alcohol having 1 to 6 carbon atoms and a divalent metal ion in an aqueous solvent, wherein the concentration of the divalent metal ion is from about 6 mmol/L to about 82 mmol/L.