Cell Substance Introduction via Rapid Pressure Pulsing
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Solution Overview
Problem
Existing methods for introducing substances into cells are inefficient and time-consuming, necessitating a technology that can easily and quickly deliver substances into cells with high efficiency.
Innovation Solution
A device comprising an accommodation unit for cells and substances, with a driving unit for rapid pressurization, achieving pressure increase within 2.0 msec or less and pressures between 0.10 MPa and 35 MPa, preferably using a gas in the accommodation unit.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional methods such as electroporation, lipofection, or gene gun are used to introduce substances into cells, then the substance can be delivered into cells, but the process is time-consuming and has low efficiency
Solution Approach 1:
The patent employs periodic pressure changes (pressurization followed by depressurization) to introduce substances into cells. The pressurization phase forces the substance into the cell, while the subsequent depressurization phase allows cell recovery, creating an efficient cyclic process that achieves high introduction efficiency in short time
Solution Approach 2:
The patent utilizes dynamic pressure parameter changes as the core mechanism. By rapidly changing pressure from negative to positive and back to negative, the system achieves efficient substance introduction without the time-consuming procedures of traditional methods, directly addressing the productivity-time contradiction
2Productivity
If high pressure is applied to introduce substance into cells rapidly, then introduction efficiency increases, but cell damage may occur
Solution Approach 1:
The patent incorporates a depressurization phase after pressurization to allow cells to recover from the high-pressure stress. This cushioning period prevents permanent cell damage while maintaining the efficiency benefits of rapid pressurization, resolving the contradiction between introduction speed and cell safety
Solution Approach 2:
The cyclic nature of pressurization-depressurization allows the system to apply high pressure briefly for efficient introduction, then return to negative pressure for cell recovery. This periodic action pattern enables high productivity without causing harmful permanent damage to cells
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device enables efficient and rapid introduction of substances into cells, particularly into the cytoplasm or nucleus, with high efficiency and minimal cell damage.
Implementation Method 1
a driving unit for pressurization of the solution, wherein regarding the pressurization of the solution, time from when the pressurization starts until pressure reaches a maximum pressure is 2.0 msec or shorter
Data Source
AI summary
Proposed is a device that can easily introduce a substance into cells in vitro with high efficiency and in a short time. The device may include an accommodation unit for accommodating a solution containing the cells and the substance. The device may also include a driving unit for pressurization of the solution. Regarding the pressurization of the solution, time from when the pressurization starts until pressure reaches a maximum pressure may be 2.0 msec or shorter.