Cell Printing Apparatus Heated Liquid Supply

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Solution Overview

Problem

Conventional cell printing methods face challenges in maintaining the liquid cell substance in a cured state during 3D structure lamination, particularly due to inadequate heat transfer and risk of structure collapse, and contamination issues with liquid bath methods.

Innovation Solution

A cell printing apparatus with a nozzle for discharging a liquid cell substance, a container for lamination, and a supply unit that provides heated liquid to ensure the liquid cell substance reaches gelation temperature, along with a heating unit for maintaining the container temperature, allowing simultaneous curing during lamination.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Temperature

If a heat source is provided under the bottom to heat the liquid cell substance to gelation temperature, then the liquid cell substance can be cured, but when the structure becomes higher, the heat cannot be properly transferred to the upper layer

Engineering Contradiction:
Improvegelation temperatureVSAvoidheat transfer efficiency
Core Design Contradiction:
TemperatureVSLoss of energy

Solution Approach 1:

The invention transitions from bottom-up heating (one-dimensional heat transfer through the height of the structure) to top-down heating (heat application from above the liquid cell substance). This dimensional change in heat application direction allows the heated liquid to directly contact and warm the upper layers of the 3D structure, solving the heat transfer inefficiency in tall structures.

Inventive Principle:
Principle #17Another dimension (Dimensionality change)

2Ease of operation

If the liquid cell substance is discharged at low temperature to maintain liquid state, then it can be discharged smoothly, but the layers slip down and the laminated structure collapses

Engineering Contradiction:
Improvedischarge smoothnessVSAvoidstructural stability
Core Design Contradiction:
Ease of operationVSStability of the object's composition

Solution Approach 1:

The invention applies heating action before and during the lamination process by supplying heated liquid to the container. This preliminary and concurrent heating ensures that the liquid cell substance reaches gelation temperature and cures in place, preventing layer slippage and structural collapse while maintaining discharge smoothness at lower temperatures.

Inventive Principle:
Principle #10Preliminary action

3Temperature

If a liquid bath is used for cell printing, then the liquid cell substance can be maintained, but cell contamination risk increases and cell layers do not properly adhere to the bottom

Engineering Contradiction:
Improveliquid state maintenanceVSAvoidcontamination risk
Core Design Contradiction:
TemperatureVSObject-affected harmful factors

Solution Approach 1:

The invention extracts and removes the liquid bath environment from the cell printing process. Instead of printing in a liquid bath, the invention uses a dry container where heated liquid is supplied directly to the container. This extraction eliminates the contamination risks associated with liquid baths while maintaining proper cell layer adhesion to the bottom through controlled heating and curing.

Inventive Principle:
Principle #2Taking out (Extraction)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables the successful fabrication of 3D structures by maintaining the liquid cell substance in a cured state throughout the lamination process, reducing the risk of structure collapse and contamination, and ensuring precise 3D structure formation.

Implementation Method 1

a supply unit for supplying a heated liquid to the container

Methodology Applied
Scientific EffectHeating: Heating

Implementation Method 2

The liquid cell substance is maintained in a liquid state at a temperature lower than a first temperature but is cured at a temperature of a second temperature or higher

Methodology Applied
Scientific EffectGelation: Gel

Implementation Method 3

a heating unit for heating the container

Methodology Applied
Scientific EffectHeating: Heating

Data Source

PatentUS11015163B2Cell printing apparatus
Publication Date: 2021.05.25 T&R BIOFAB CO LTD
  • US11015163B2 patent drawing
  • US11015163B2 patent drawing
  • US11015163B2 patent drawing

AI summary

A cell printing apparatus according to the present disclosure comprises: a nozzle through which a liquid cell substance is discharged; a container in which the liquid cell substance discharged through the nozzle is laminated into a three-dimensional structure; and a supply unit for supplying the liquid heated to a predetermined temperature to the container.