Automated Cell Processing System with Integrated Quantification
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Solution Overview
Problem
Current tissue processing methods for stem cell extraction are cumbersome, time-consuming, and result in low cell viability, often requiring external measurements and interrupting sterile processes.
Innovation Solution
An automated system for tissue-derived cell processing that includes a cell quantifying component for measuring cell quantity and quality using impedance, optical, and fluidic methods, integrated with a centrifuge and enzyme management system to optimize processing parameters based on real-time data.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual centrifugation and external measurement methods are used, then processing flexibility is maintained, but processing time increases and cell viability decreases
Solution Approach 1:
The patent integrates the cell quantifying component directly into the centrifuge system, merging measurement and processing functions into a single automated unit. This eliminates the need for external measurements and manual handling, thereby reducing processing time while maintaining cell viability through continuous automated operation.
Solution Approach 2:
The system performs self-measurement and self-adjustment of processing parameters. The cell quantifying component automatically measures cell concentration and quality, and the control unit adjusts centrifugation parameters accordingly without external intervention, enabling rapid processing while preserving cell viability through optimized parameters.
2Loss of time
If automated processing is implemented, then processing time is reduced, but system complexity increases
Solution Approach 1:
The cell quantifying component serves multiple functions: it measures cell concentration, assesses cell quality, and provides data for parameter optimization. This multi-functionality reduces the need for separate measurement devices, thereby limiting the increase in system complexity while enabling automated processing.
Solution Approach 2:
The system implements a feedback loop where the cell quantifying component continuously monitors cell parameters and the control unit adjusts processing parameters in real-time. This automated feedback mechanism enables rapid processing without requiring complex manual intervention systems.
3Reliability
If external measurements are performed, then processing flexibility is maintained, but sterile process integrity is compromised
Solution Approach 1:
By integrating the cell quantifying component within the closed centrifuge system, the patent eliminates the need to open the sterile environment for measurements. The measurement and processing functions are combined in a way that maintains sterile integrity while providing automated operation.
Solution Approach 2:
The system performs all measurements and adjustments internally without external intervention. The cell quantifying component and control unit work together to automatically monitor and optimize processing while maintaining the closed sterile environment, thereby preserving sterile integrity with simple operation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient, sterile, and high-quality stem cell processing by reducing mechanical damage, improving cell viability, and eliminating the need for external measurements, allowing for optimized processing parameters and enhanced clinical application.
Implementation Method 1
The cell quantifying component (13a, 23a) for quantifying the volume flow in the system
Implementation Method 2
The cell quantifying component (13a, 23a) for quantifying the volume flow in the system
Implementation Method 3
The centrifuge component (26)
Implementation Method 4
an enzyme component configured to input at least one enzyme in the AMFAT component (11) via the enzyme inlet (22)
Data Source
AI summary
The present invention is directed to a system, method, use, and diagnostic kit for cell processing. The invention discloses a cell-quantifying component configured for quantifying the cells in the derived tissue. The cell quantifying component can be configured for quantifying the volume flow in the system. Based on the cell-relevant data generated by at least cell quantification, the operational parameters for cell processing are determined.