Cell Processing Apparatus Using Shear Force for Target Substance Introduction
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Solution Overview
Problem
Existing cell processing methods for introducing target substances into cells, such as those described in Japanese Patent No. 5645657, face limitations in efficiency and versatility, particularly in selecting cells for processing and applying shear forces effectively.
Innovation Solution
A cell processing method and apparatus that select cells with diameters 1.3 or more times the mode of the cell diameter distribution for processing, utilizing a shear force applied through an orifice in a liquid ejection head to introduce target substances into cells, enhancing the efficiency and versatility of the process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If cells are selected based on larger diameter (1.3 or more times the mode) from a cell group including cells in proliferation process, then the efficiency of target substance introduction is significantly increased, but the device complexity increases due to the need for cell selection mechanism
Solution Approach 1:
The patent applies preliminary action by selecting cells with larger diameters (1.3 or more times the mode) from the cell group before the target substance introduction process. This pre-selection based on cell diameter ensures that only cells in a suitable state for high-efficiency introduction are processed, thereby achieving significantly improved introduction efficiency while managing device complexity through a focused selection criterion
2Adaptability or versatility
If shear force is applied through liquid ejection head with orifice to introduce target substance into cell, then the versatility for usable target substances is improved, but the harmful factors to cells increase due to shear force application
Solution Approach 1:
The patent applies parameter changes by utilizing shear force generated through the liquid ejection head's orifice to introduce target substances into cells. By controlling the liquid ejection parameters (flow rate, pressure, orifice size), the system achieves high versatility for different target substances while managing the harmful effects of shear force through optimized ejection conditions that balance introduction efficiency with cell integrity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method significantly increases the efficiency of target substance introduction into cells, particularly during proliferation processes, by selectively targeting cells with larger diameters and using a shear force application mechanism, resulting in higher introduction ratios compared to conventional methods.
Implementation Method 1
an introducing step of introducing the target substance into the cell to be processed by allowing a shear force to act on the cell to be processed
Data Source
AI summary
Provided is a cell processing method including: preparing a cell suspension containing a cell to be processed and a target substance; and introducing the target substance into the cell to be processed by allowing a shear force to act on the cell to be processed, wherein the cell to be processed is a cell, which is selected from a cell group including cells in a proliferation process, and which has a cell diameter larger than a mode in a cell diameter distribution of the cell group.