Cell Rejuvenation Factor Composition Without c-Myc Integration

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Solution Overview

Problem

Existing combinations of reprogramming factors for rejuvenating cells are limited and require safety improvements to effectively reverse aging and restore cellular viability.

Innovation Solution

A composition comprising factors such as SOX1, SOX2, SOX3, GMNN, GATA3, and GATA6, along with Klf4, is used to restore cellular viability by altering transcription and promoting cellular rejuvenation without integrating exogenous DNA into the genome.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If existing combinations of reprogramming factors (Oct4, Sox2, Klf4, c-Myc) are used to rejuvenate cells, then cellular viability is improved, but safety concerns arise and the combination is limited

Engineering Contradiction:

Improvecellular viabilityVSAvoidsafety concerns

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts and removes the potentially harmful c-Myc factor from the traditional Yamanaka combination, replacing it with safer alternative factors (Sox1/Sox3, Gata3/Gata6, or Klf4) that achieve similar rejuvenation effects without the same safety concerns. This extraction resolves the contradiction by maintaining cellular viability while eliminating harmful effects.

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent changes the parameters of the reprogramming factor combination by substituting c-Myc with alternative factors having different safety profiles. This parameter change allows achieving the same rejuvenation function with improved safety characteristics, resolving the technical contradiction between viability and safety.

Inventive Principle:

Principle #35Parameter changes

2Reliability

If existing reprogramming factors are used to reverse aging, then cellular functions are improved, but the combination is relatively limited

Engineering Contradiction:

Improvecellular functionsVSAvoidcombination variety

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent demonstrates that multiple different factor combinations (Sox1/Sox3, Gata3/Gata6, Klf4) can universally achieve cell rejuvenation and functional restoration. This multi-functionality expands the versatility of reprogramming approaches while maintaining effective cellular function restoration, resolving the contradiction between functional improvement and combination variety.

Inventive Principle:

Principle #6Universality (Multi-functionality)

3Reliability

If complete reprogramming is achieved to restore cellular viability, then aging is reversed, but teratoma formation risk increases

Engineering Contradiction:

Improvecellular viabilityVSAvoidteratoma formation

Core Design Contradiction:

ReliabilityVSObject-generated harmful factors

Solution Approach 1:

The patent applies partial reprogramming action by using limited combinations of factors (Sox1/Sox3, Gata3/Gata6, Klf4) that are sufficient to achieve cellular rejuvenation and functional restoration without completing the full reprogramming process. This partial action resolves the contradiction by achieving viable cellular restoration while avoiding the harmful teratoma formation associated with complete reprogramming.

Inventive Principle:

Principle #16Partial or excessive action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The composition effectively reverses aging, promotes cellular regeneration, and enhances the functional capacity of cells, tissues, and organs by restoring epigenetic information and reducing aging markers, while avoiding teratoma formation and complete reprogramming.

Implementation Method 1

Methodology Applied

Scientific EffectTranscription factor reprogramming:

Data Source

PatentUS20260014228A1Composition and use method therefor

Publication Date: 2026.01.15 NANJING REJU THERAPEUTICS INC

AI summary

The present invention provides a composition based on three factors and used for restoring viability of cells, tissue, or organs of a subject, and a method for using the composition to restore viability of the cells, tissue, or organs of the subject. By means of the composition and the method, the viability or functions of the cells, tissue, or organs of the subject can be restored. The composition and the method can be used for any disease or damage that requires repairing or replacement of malfunctional tissue.