Cell Selection Chromatography with Thermal and Sterile Gas Control
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Solution Overview
Problem
Existing devices and methods for generating cell populations for cell therapy, such as those involving immune cells like T cells, are inefficient and require improvements to enhance their suitability and speed.
Innovation Solution
A housing assembly for column chromatography that includes a temperature control member to regulate the stationary phase temperature and a connector to introduce gas, along with a chromatography column jacket member for temperature maintenance and gas sterility, facilitating efficient cell selection and stimulation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If gas is introduced into the chromatography column to improve cell selection and stimulation efficiency, then productivity is improved, but maintaining gas sterility and proper temperature control becomes more difficult
Solution Approach 1:
The patent introduces gas (such as carbon dioxide) into the chromatography column to create a controlled atmosphere that enhances cell selection and stimulation. The gas delivery system is designed to maintain sterility while improving productivity, resolving the contradiction between faster cell generation and maintaining reliable sterile conditions.
Solution Approach 2:
The chromatography column assembly integrates multiple functions: temperature control through the jacket, gas delivery through the stationary phase, and cell selection/stimulation. This multi-functional design allows simultaneous optimization of temperature control and gas sterility while maintaining high productivity.
2Manufacturing precision
If temperature control is enhanced to optimize cell stimulation, then manufacturing precision is improved, but device complexity increases
Solution Approach 1:
The patent uses a temperature control jacket as an intermediary system that surrounds the chromatography column. This jacket provides precise temperature control through a simple external structure, avoiding the need for complex internal temperature control mechanisms within the column itself.
Solution Approach 2:
The temperature control system is segmented into an external jacket that is separate from the main chromatography column. This segmentation allows independent optimization of temperature control without complicating the column structure, maintaining manufacturing precision while managing device complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The assembly significantly reduces the time required to generate selected and stimulated cells for genetic engineering and cell therapy by optimizing temperature control and gas sterility, enhancing the efficiency and effectiveness of the cell preparation process.
Implementation Method 1
a temperature control member configured to provide heat to the stationary phase in the internal cavity
Implementation Method 2
a connector configured to operably connect the internal cavity to a gas source, thereby permitting or effecting intake of gas into the internal cavity
Data Source
AI summary
Provided herein are devices and methods for selecting and stimulating a plurality of cells in a sample of cells using column chromatography. In some aspects. provided is a device comprising a temperature control member to provide heat to a chromatography stationary phase and a connector configured to provide air to the stationary phase during column chromatography. In some aspects. the devices and methods provided herein reduce the time needed to generate a population of selected and stimulated cells useful for genetic engineering. and ultimately, cell therapy. compared to existing devices and methods.