Cell Separation Container With Air Pressure Control
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Solution Overview
Problem
Existing cell separation devices require large amounts of dispersant-containing liquid, leading to inefficiencies in the cell dispersion process, and manual operations are labor-intensive in cell culture techniques.
Innovation Solution
A cell separation container with a collection chamber, a tissue holding chamber, and a filter, equipped with an air pressure adjusting mechanism that allows for efficient use and ejection of treatment liquid by controlling gas inflow and outflow, minimizing the amount of liquid needed and simplifying manual operations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If a large amount of dispersant-containing liquid is stored in the liquid storage member, then the cell dispersion treatment can be performed, but the efficiency of the device decreases
Solution Approach 1:
The device is divided into two separate chambers: a tissue holding chamber for containing the tissue and a collection chamber for collecting the dispersant-containing liquid. The filter separates these chambers, allowing the liquid to be collected in the collection chamber while the tissue remains in the tissue holding chamber. This segmentation enables efficient liquid collection and reuse, reducing the overall amount of dispersant needed.
Solution Approach 2:
The filter is designed to retain the dispersant-containing liquid while allowing cells to pass through. The collected liquid in the collection chamber can be reused for subsequent treatments, reducing waste and the total amount of dispersant required. This recovery mechanism directly addresses the efficiency problem by minimizing material consumption.
2Ease of operation
If manual operations are used for cell separation, then the process can be performed, but the operational burden on workers increases
Solution Approach 1:
The filter automatically separates cells from the dispersant-containing liquid based on size differences. Cells pass through the filter into the collection chamber while the liquid is retained, eliminating the need for manual separation operations. This self-service mechanism simplifies the operational process while maintaining ease of use.
Solution Approach 2:
The device utilizes pressure differential created by pouring the dispersant-containing liquid over the tissue to drive the separation process. The liquid flows through the filter under gravity and pressure, automatically separating cells from the liquid without requiring complex mechanical or manual intervention.
3Ease of operation
If the treatment liquid is poured into the tissue holding chamber, then the cell treatment can be performed, but the treatment liquid cannot be easily ejected
Solution Approach 1:
The collection chamber is positioned below the tissue holding chamber and is connected through the filter. When the tissue holding chamber is tilted or inverted, gravity causes the treatment liquid to flow through the filter into the collection chamber, enabling easy ejection. This segmented design with gravitational assistance simplifies liquid disposal.
Solution Approach 2:
The device allows easy ejection of treatment liquid by inverting the tissue holding chamber. The liquid flows downward through the filter into the collection chamber due to gravity, reversing the normal pouring direction. This inversion mechanism provides simple and efficient liquid ejection without complex valves or pumps.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient cell separation with minimal treatment liquid usage and simplifies the process, reducing the operational burden on workers by allowing precise control over the treatment liquid's presence and ejection, thus improving the efficiency of cell separation treatments.
Implementation Method 1
a filter (40) disposed as the entirety or part of a structure for partitioning the collection chamber (20) and the tissue holding chamber (30) from each other, the filter (40) being capable of holding the tissue and allowing the cell to pass therethrough
Implementation Method 2
an air pressure adjusting mechanism capable of switching between a process of holding a treatment liquid poured into the tissue holding chamber without causing the treatment liquid to pass through the filter and a process of ejecting a treatment liquid poured into the tissue holding chamber to the collection chamber by causing the treatment liquid to pass through the filter, the switching being achieved by adjusting inflow and outflow of gases in the collection chamber and the tissue holding chamber
Data Source
Figure 1
Figure 2
Figure 3~4
AI summary
A cell separation container 10 includes a collection chamber 20 and a tissue holding chamber 30 which are partitioned by a filter 40. The cell separation container 10 includes a first air pressure adjuster 60 configured to adjust the inflow and outflow of gas in the collection chamber 20 and a second air pressure adjuster 70 configured to adjust the inflow and outflow of gas in the tissue holding chamber 30. Thus, the inflow and outflow of the outside air and the gases in the collection chamber 20 and the tissue holding chamber 30 can be adjusted. In the cell separation container 10, switching between holding a treatment liquid in the tissue holding chamber 30 and ejecting a treatment liquid to the collection chamber 20 can be performed by adjusting the inflow and outflow of the outside air and the gas in the connection chamber 20.