Cell Sheet Detachment Using Multidirectional Tapping and Shaking
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Solution Overview
Problem
Existing methods for detaching cell sheets from culture containers often result in tearing and inefficiency, particularly when the direction of impact is parallel to the culture surface.
Innovation Solution
A method involving a combination of tapping and shaking processes, where the directions of tapping and shaking are perpendicular to each other, is used to detach cell sheets, utilizing external stimuli like tapping, shaking, and ultrasonic waves to efficiently reduce tearing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If a single direction tapping or shaking method is used to detach cell sheets, then the detachment process is simple, but the cell sheets suffer from tearing and the detachment efficiency is low
Solution Approach 1:
The patent applies multi-directional tapping and shaking (changing the dimension of detachment force application from single-direction to multi-directional) to reduce cell sheet tearing while improving detachment efficiency. Specifically, tapping is applied in a first direction and shaking in a second direction that differs from the first direction when viewed perpendicular to the culture surface, creating comprehensive detachment forces that minimize stress concentration on the cell sheet.
Solution Approach 2:
The patent employs periodic alternating action between tapping and shaking processes. The tapping process and shaking process are performed in sequence or combination, creating periodic mechanical stimuli that progressively detach the cell sheet without causing excessive stress in any single direction, thereby reducing tearing while maintaining high detachment efficiency.
2Loss of time
If strong mechanical force is applied to accelerate cell sheet detachment, then the detachment time is reduced, but the cell sheet quality deteriorates with increased tearing
Solution Approach 1:
By applying mechanical forces in multiple directions (tapping in first direction, shaking in second direction) rather than concentrating force in a single direction, the patent achieves effective detachment without requiring excessive force magnitude. This multi-dimensional approach distributes the mechanical stress, preventing cell sheet tearing while maintaining rapid detachment.
Solution Approach 2:
The patent uses dynamic mechanical actions (tapping followed by shaking) rather than static or continuous single-mode force application. The combination of impulsive tapping and oscillatory shaking creates a dynamic detachment process that is both time-efficient and gentle on the cell sheet structure, preserving quality while reducing detachment time.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively detaches cell sheets while minimizing tearing and reducing detachment time, maintaining high cell survival rates.
Implementation Method 1
a tapping process of tapping the culture container in a first direction that contains a direction component parallel to the culture surface
Implementation Method 2
a shaking process of shaking the culture container in a second direction that contains a direction component parallel to the culture surface
Data Source
AI summary
A cell detachment method that detaches a cell sheet adhering to a culture surface of a culture container from the culture container by using a cell detachment system includes a tapping process of tapping the culture container in a first direction that contains a direction component parallel to the culture surface; and a shaking process of shaking the culture container in a second direction that contains a direction component parallel to the culture surface, in which the first direction and the second direction are different from each other as viewed in a direction perpendicular to the culture surface.