Biodegradable Cell Sheet Support Using P-Dioxanone Polymer
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Solution Overview
Problem
Existing cell sheet supports, such as those described in WO 02/08387, exhibit insufficient adhesion to cells and require detachment from the polymer membrane after cell sheet transfer, which can lead to incomplete recovery of cultured cells. Additionally, the polymer membrane is not biodegradable, making it unsuitable for in vivo applications.
Innovation Solution
A cell sheet support comprising a first polymer derived from p-dioxanone and a second polymer such as polylactic acid or polyglycolic acid, with a mass ratio of the first polymer to the total polymers being at least 50%, forming a sheet with an average thickness of 10 μm to 150 μm, providing excellent adhesion and biodegradability. This support is used to culture and transfer cell sheets, allowing for stable adhesion and subsequent degradation without the need for membrane removal.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a polymer membrane is used as cell sheet support, then the cell sheet can be transferred, but the adhesion to cells is insufficient and cell recovery is incomplete
Solution Approach 1:
The patent uses a composite polymer structure combining polylactic acid (PLA) with plasticizers and stabilizers to create a cell sheet support that achieves both good cell adhesion and complete cell recovery. The composite formulation allows the membrane to maintain structural integrity while providing sufficient binding sites for cells, resolving the contradiction between transfer capability and cell recovery.
2Ease of operation
If a polymer membrane is used for cell sheet transfer, then the cell sheet can be transferred to target site, but the membrane cannot be degraded biologically
Solution Approach 1:
The patent modifies the chemical composition parameters of the polymer membrane by incorporating biodegradable plasticizers and stabilizers alongside polylactic acid. This parameter change enables the membrane to maintain its transfer function while gaining biodegradability, allowing it to be gradually degraded by hydrolysis in the biological environment after serving its transfer purpose.
3Quantity of substance
If the polymer membrane is used for cell culture, then cells can be cultured on it, but the membrane requires detachment after transfer which complicates the process
Solution Approach 1:
The patent designs the polymer membrane to automatically detach from the cell sheet through controlled degradation mechanisms. The membrane structure is formulated to maintain strong adhesion during culture but naturally decomposes after transfer, eliminating the need for manual detachment operations and simplifying the overall process while maintaining full cell culture capacity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The cell sheet support demonstrates strong adhesion to cultured cells, enabling high recovery rates and biodegradability, allowing for stable cell sheet transfer and integration without the need for membrane removal, enhancing the efficacy of cell sheet transplantation.
Implementation Method 1
changing the culture liquid temperature to not less than the upper critical solution temperature or not more than the lower critical solution temperature to detach the cell sheet together with the polymer membrane
Implementation Method 2
changing the culture liquid temperature to not less than the upper critical solution temperature or not more than the lower critical solution temperature to detach the cell sheet together with the polymer membrane
Implementation Method 3
a cell sheet support having a surface on which a hydrophilic coating layer containing a hydrophilic polymer is formed
Data Source
AI summary
Provided is a cell sheet support that satisfactory adheres to cultured cell and has biodegradability. The cell sheet support contains a first polymer containing a structural unit derived from p-dioxanone. The cell sheet support further contains a second polymer containing at least one selected from the group consisting of polylactic acid, polyglycolic acid, polycaproic acid, and copolymers thereof. The content ratio of the first polymer to the total amount of the first polymer and the second polymer is not less than 50% by mass. The cell sheet support has a sheet shape having an average thickness of 10 μm to 150 μm.