Cell Sheet Preservation Medium for Viability and Adhesion Retention
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Solution Overview
Problem
Existing cell sheet preservation solutions fail to maintain cell viability, secreted factors, and intercellular connections during storage and transportation, affecting their clinical application.
Innovation Solution
A cell sheet protection solution comprising a buffered salt solution, extracellular matrix components, reducing agents, substances for cell metabolism, and hormones, which includes specific combinations such as PBS with gelatin and vitamin C, or Hanks buffer with fibronectin and sodium selenate, to maintain cell integrity and function.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional preservation solutions are used for cell sheet storage and transportation, then the storage and transportation process can be completed, but the cell viability, secreted factors, and intercellular connections are compromised
Solution Approach 1:
The patent modifies the chemical composition parameters of the preservation solution by incorporating specific concentrations of buffered salt solution (pH 7.2-7.4), extracellular matrix components (0.1-10 mg/mL), reducing agents (1-10 mM), metabolic substrates, and growth factors. These parameter changes create an optimized chemical environment that maintains cell viability and prevents apoptosis during storage and transportation.
Solution Approach 2:
The preservation solution is formulated as a composite system integrating multiple functional components: buffered salt solution for pH and osmotic balance, extracellular matrix components for structural support and cell adhesion, reducing agents for antioxidant protection, metabolic substrates for energy supply, and growth factors for cell survival signaling. This composite formulation synergistically addresses multiple harmful effects simultaneously.
2Stability of the object's composition
If cell sheets are stored and transported without proper protection solution, then the process is simple, but the integrity of cell sheet and cell connections is destroyed
Solution Approach 1:
The preservation solution is designed as a multi-functional medium that simultaneously performs pH buffering, osmotic regulation, antioxidant protection, nutrient supply, and structural support functions. This universal solution eliminates the need for multiple separate preservation systems while maintaining comprehensive cell sheet integrity.
Solution Approach 2:
The preservation solution is prepared in advance with all necessary components at optimized concentrations before cell sheet storage. The extracellular matrix components and protective agents are pre-incorporated to create a ready-to-use formulation that immediately provides protection upon contact with the cell sheet, eliminating the need for complex step-by-step preparation during storage operations.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution effectively preserves cell viability, secreted cytokines, and intercellular connections, ensuring the cell sheet's integrity and functionality during storage and transportation, as demonstrated by maintained cytokine secretion and apoptosis reduction.
Implementation Method 1
c. a reducing agent
Implementation Method 2
b. an extracellular matrix component
Data Source
AI summary
The present disclosure relates to a cell sheet protection solution, such as a stem cell sheet protection solution, such as a mesenchymal stem cell sheet protection solution. The present disclosure also relates to use of the cell sheet protection solution in storage and transportation of the cell sheet.