Labelled Cell Stress Markers for Early Angiogenesis Detection
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Solution Overview
Problem
Current diagnosis of wet-AMD relies on imaging the retina, often resulting in late detection due to its silent and rapidly progressive nature, leading to ineffective treatment and significant morbidity.
Innovation Solution
A labelled cell stress marker, such as annexin or its variants, is used to identify stressed cells at a single-cell level, predicting early-stage angiogenesis by binding to phosphatidylserine on endothelial cell membranes, allowing for early clinical intervention.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If retinal imaging is used for diagnosis of wet-AMD, then diagnosis can be performed, but detection is delayed until later stages due to the silent and rapidly progressive nature of the disease
Solution Approach 1:
The patent applies preliminary action by using labelled cell stress markers to detect and predict angiogenesis before it progresses to clinically apparent wet-AMD. The marker identifies stressed endothelial cells at the single-cell level, enabling early detection and prediction of disease development before traditional retinal imaging can detect abnormalities, thus allowing treatment to be administered at an unexpectedly early stage
2Measurement precision
If traditional retinal imaging is used, then existing blood vessels can be visualized, but early-stage angiogenesis cannot be detected
Solution Approach 1:
The patent applies local quality by focusing detection at the single-cell level rather than requiring visible structural changes in the retina. The labelled cell stress marker binds specifically to phosphatidylserine on the membrane of individual stressed endothelial cells, enabling detection of local cellular stress events that precede and predict angiogenesis, without requiring the angiogenesis to be visually apparent in retinal images
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables early identification and prediction of angiogenesis, preventing rapid symptom progression by allowing timely clinical intervention, particularly in conditions like wet-AMD.
Implementation Method 1
The cell stress marker is also capable of labelling apoptosing cells... the marker may be known as a compound or molecule that specifically binds to stressed cells
Data Source
AI summary
The present invention provides a labelled cell stress marker for use in identifying or predicting angiogenesis in a subject. Also provided is a method of identifying or predicting angiogenesis in a subject, the subject having been administered a labelled cell stress marker. The method comprises the steps of (a) generating an image from the subject using a first imaging device and (b) identifying labelled cell stress marker positive cell(s) in the image. Angiogenesis is identified or predicted if labelled cell stress marker positive cell(s) are identified in the image.