Cell Delivery Syringe Loading to Prevent Needle Clogging
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Solution Overview
Problem
Existing methods for delivering high-density cell suspensions, such as those containing mesenchymal stem cells transiently-transfected by a polynucleotide encoding a Notch intracellular domain, face challenges like needle blockage and cell damage due to high viscosity, leading to inconsistent treatment results.
Innovation Solution
A method involving a syringe with a chemically inert polymeric plunger tip and a horizontal alignment for loading cell suspensions, followed by use of a cannula or needle to expel controlled droplets, ensuring uniform distribution and minimizing cell damage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a fine needle is used to deliver high-density cell suspensions, then precision of delivery is improved, but needle blockage occurs due to high viscosity
Solution Approach 1:
The syringe barrel is divided into multiple lumens (first lumen and second lumen) with different functions. The first lumen receives high-density cell suspension while the second lumen receives lower-density suspension, allowing segmented delivery that prevents blockage in the fine needle while maintaining delivery precision.
Solution Approach 2:
A plunger with a porous tip acts as an intermediary between the lumens and the needle. The porous structure allows cell suspension to pass through while filtering out clumps that would cause blockage, enabling precise delivery without needle obstruction.
2Productivity
If high concentration of cells is administered, then treatment effectiveness is improved, but cell damage occurs due to needle blockage
Solution Approach 1:
Cell suspension is segmented into multiple lumens with different cell densities. The first lumen contains high-density suspension for effectiveness, while the second lumen contains filtered suspension that prevents cell damage from blockage, allowing both effectiveness and safety to be achieved simultaneously.
Solution Approach 2:
The porous plunger tip serves as a protective intermediary that filters the cell suspension before it enters the needle. This prevents cell clumps from causing blockage and damaging the needle, thereby preserving cell viability and treatment effectiveness.
3Reliability
If high-density cell suspension is delivered, then treatment consistency is improved, but shear stress on cells increases
Solution Approach 1:
The system segments cell suspension delivery into multiple lumens, allowing the fine needle to receive only the filtered portion that causes minimal shear stress. This maintains treatment consistency through controlled delivery while reducing mechanical stress on cells.
Solution Approach 2:
The porous plunger tip acts as a gentle intermediary that filters cell suspension without applying excessive shear stress. This allows consistent delivery of therapeutic cells while minimizing mechanical damage from high-density suspension injection.
Data Source
AI summary
A method of preparing a syringe in connection with a therapeutic treatment is disclosed. The method can include removing a plunger of the syringe from a barrel of the syringe, aligning the barrel in a horizontal orientation, filling a lumen of the barrel with a viscous material through an opening at a proximal end of the barrel, and inserting a plunger tip into the lumen to seal the lumen. The method can also include attaching an implantation device to a hub coupled to the barrel at a distal end of the barrel. The method can also include depressing the plunger until the cell suspension fills the implantation device and a droplet of the cell suspension is expelled from a distal tip of the implantation device.