Cell Tissue Solution Placement for Stable Well Coverage

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Solution Overview

Problem

Existing methods for culturing cell tissues in a container face issues with the adherence of application solutions to the well walls due to static electricity, leading to incomplete coverage and potential collapse of the first application solution, which affects reproducibility and accuracy in cell tissue production.

Innovation Solution

A method and device that supply first and second application solutions with precise positioning, ensuring the distance and dimensions between them satisfy specific conditions (Dn, m < r2m - r1n or Dn, m > r2m + r1n) to accurately cover the first solution, using a mechanism that allows simultaneous and sequential application across a plate of containers.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If the second application solution is dripped close to the inner wall of the well using a dispenser, then the application process can be completed, but the second application solution adheres to the inner wall with static electricity or deviates from the target position, causing incomplete coverage and collapse of the first application solution

Engineering Contradiction:

Improveapplication process completionVSAvoidposition accuracy of application solution

Core Design Contradiction:

ProductivityVSManufacturing precision

Solution Approach 1:

The patent calculates the optimal dripping position of the second application solution in advance, considering the radius of the first application solution (r1), the radius of the second application solution (r2), and the distance from the well center to the inner wall (R). By pre-determining the position where the second solution will be dripped to ensure it covers the first solution without adhering to the wall, the method prevents position deviation and incomplete coverage before they occur.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent establishes a quantitative relationship between the parameters (r1, r2, R) and the optimal dripping position, creating a feedback mechanism where the dripping position is adjusted based on the measured or known dimensions of the application solutions and well. This ensures that the second application solution is always dripped at the correct position to achieve complete coverage without wall adhesion.

Inventive Principle:

Principle #23Feedback

2Device complexity

If the second application solution is supplied without precise positioning control, then the application process is simpler, but the first application solution may not be completely covered or may collapse

Engineering Contradiction:

Improveapplication mechanism complexityVSAvoidcoverage completeness of application solution

Core Design Contradiction:

Device complexityVSReliability

Solution Approach 1:

The patent focuses on controlling the positional parameter of the second application solution relative to the first application solution. By establishing the quantitative relationship D ≥ r1 + r2 (where D is the distance from well center to dripping position), the method ensures reliable coverage without requiring complex device modifications, only precise positioning based on parameter control.

Inventive Principle:

Principle #35Parameter changes

3Loss of time

If multiple application solutions are supplied sequentially without coordinated positioning, then the production time is longer, but position accuracy can be ensured, whereas simultaneous supply reduces time but requires precise coordination

Engineering Contradiction:

Improvetotal production timeVSAvoidcoordination mechanism complexity

Core Design Contradiction:

Loss of timeVSDevice complexity

Solution Approach 1:

The patent combines the positioning control of the first and second application solutions into a unified coordinate system centered on the well. By establishing the relationship between the dripping position and the dimensions of both solutions (using parameters r1, r2, and R), the method enables coordinated supply that reduces total production time while maintaining position accuracy through a single reference framework.

Inventive Principle:

Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach enhances reproducibility and accuracy in cell tissue production with high throughput and reduced production time by ensuring precise application solution positioning and coverage, addressing the issues of adherence and incomplete coverage in previous methods.

Implementation Method 1

the second application solution adheres to the inner wall of the well with static electricity

Methodology Applied

Scientific EffectStatic electricity: Electrostatics

Data Source

PatentUS20260078346A1Cell Tissue Production Method, Application Method and Application Device

Publication Date: 2026.03.19 NTN CORP

AI summary

If a first dimension of each of N first application solutions is represented as r1n (n is a natural number of 1≤n≤N), a second dimension of each of M second application solutions is represented as r2m (m is a natural number of 1≤m≤M), and the distance between the center of any first application solution, a first dimension of which is r1n, selected out of the N first application solutions and the center of any second application solution, a second dimension of which is r2m, selected out of the M second application solutions is represented as Dn, m, the any first application solution and the any second application solution are supplied such that one of(Math. 1)Dn, m<r2⁢m-r1⁢n(1)and(Math. 2)Dn, m>r2⁢m+r1⁢n(2)holds between the any first application solution and the any second application solution.