Automated Cell Vitrification Device with Pre-freezing and Straw Handling
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Solution Overview
Problem
The current vitrification freezing treatment technique for cells is inefficient and labor-intensive, relying on manual operation, which is time-consuming and prone to errors, and poses safety risks due to long-term exposure to liquid nitrogen.
Innovation Solution
A vitrification freezing treatment device that automates the process using a straw device, pre-freezing device, freezing unit, driving device, and control unit, allowing for automatic cell handling and freezing, reducing manual errors and operator exposure to liquid nitrogen.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual operation is used for vitrification freezing treatment, then flexibility and adaptability are maintained, but treatment efficiency is low and labor consumption is high
Solution Approach 1:
The system performs automatic cell picking, straw insertion, and freezing operations without human intervention. The control unit coordinates the driving device to move the straw device, pre-freezing device, and freezing unit autonomously, eliminating manual labor while maintaining precise control over the vitrification process
Solution Approach 2:
Manual mechanical operations are replaced with an automated mechanical system consisting of a driving device with multiple driving parts, a controlled straw device for automatic cell pickup and transfer, and an integrated pre-freezing and freezing system. This mechanical automation resolves the contradiction by providing both high efficiency and precise control
2Loss of time
If manual operation is used for vitrification freezing treatment, then operator control is maintained, but time consumption is high and training requirements are extensive
Solution Approach 1:
The system is divided into functional modules: a driving device with first, second, and third driving parts for different movements, a straw device for cell pickup, a pre-freezing device for initial freezing, and a freezing unit for final vitrification. This segmentation allows parallel operations and reduces total processing time while making the complex system manageable through modular design
Solution Approach 2:
The pre-freezing device performs preliminary freezing of cells before they are transferred to the freezing unit for vitrification. This preliminary action prepares the cells in advance, reducing the time required for the final freezing process and overall treatment time
3Reliability
If manual operation with liquid nitrogen is used, then freezing capability is achieved, but safety risks increase due to long-time operator exposure
Solution Approach 1:
The system uses an automated straw device as an intermediary between the operator and liquid nitrogen. The straw device automatically picks up cells, transfers them through the pre-freezing device, and inserts them into the freezing unit, eliminating direct operator contact with liquid nitrogen while maintaining reliable cryopreservation through controlled freezing processes
Solution Approach 2:
The freezing unit and pre-freezing device autonomously handle the cryopreservation process using liquid nitrogen, performing self-service freezing operations without human intervention. This automation eliminates safety risks to operators while ensuring consistent and reliable cryopreservation results through precise temperature control
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device achieves high treatment efficiency and safety by automating cell siphoning, pre-freezing, and freezing processes, ensuring consistent and stable cryopreservation and thawing results independent of operator skill or condition.
Implementation Method 1
the pre-freezing device provides a freezing medium for the straws for pre-freezing the cells
Implementation Method 2
the straw device comprises straws used for siphoning cells
Data Source
AI summary
The invention discloses a vitrification freezing treatment device for cells. The vitrification freezing treatment device for cells includes a straw device, a pre-freezing device, a freezing unit, a driving device, a control unit and a carrying table used for carrying cell carriers, wherein the straw device is connected with the driving device in a driven mode, the driving device is in signal connection with the control unit, the straw device comprises straws used for obtaining cells in the cell carriers, the control unit is used for controlling the driving device to drive the straw device to obtain cells and to transfer the cells to the freezing unit, and the pre-freezing device is used for pre-freezing the straws when the straw device transfers the obtained cells to the freezing unit.