Cellular Restoration Composition via Exosome Isolation
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Solution Overview
Problem
Aging leads to decreased cellular function and chronic diseases, with cellular senescence causing inflammation and disorders, necessitating compositions and methods to improve or restore cellular health.
Innovation Solution
A method involving obtaining cell samples from donors and receivers, culturing them together in a transwell system to produce a restoring composition, which is then used to generate a restored composition that can be administered to improve cellular function and health by isolating exosomes and combining them with active agents like antimicrobials, steroids, or growth factors.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If cellular senescence is allowed to progress naturally, then cellular function deteriorates and chronic diseases increase, but aggressive interventions may cause additional cellular stress or damage
Solution Approach 1:
The patent uses young plasma as an intermediary medium to transfer restorative factors from young cells to senescent cells. The plasma contains exosomes, growth factors, and other bioactive molecules that mediate the restoration process without requiring direct cell-to-cell contact, thereby avoiding additional cellular stress while improving cellular function.
Solution Approach 2:
The patent extracts specific restorative components (exosomes, growth factors, cytokines) from young plasma and concentrates them into a therapeutic composition. This extraction allows the isolation of beneficial factors while leaving behind potential harmful or unnecessary components, enabling targeted therapy that improves cellular function without introducing extraneous stressors.
2Reliability
If traditional cellular therapy methods are used, then cellular restoration may be achieved, but the process is time-consuming and requires complex procedures
Solution Approach 1:
The patent creates a biological copy or surrogate of the restorative effects by using plasma-derived exosomes and factors that replicate the natural cell-to-cell communication and restoration processes. This copying approach bypasses the need for complex cell transplantation procedures while achieving similar restorative outcomes, significantly reducing treatment time and complexity.
Solution Approach 2:
The patent segments the complex cellular restoration process into discrete, isolatable components (exosomes, growth factors, cytokines) that can be independently purified, characterized, and administered. This segmentation allows for streamlined processing and faster preparation of therapeutic compositions compared to whole-cell therapies.
3Quantity of substance
If plasma from young donors is used directly, then restorative factors are available, but the composition may contain variable or unknown components
Solution Approach 1:
The patent extracts and isolates specific restorative components (exosomes, growth factors, cytokines) from young plasma through a series of purification steps. This extraction process removes variable or unknown components while concentrating the known beneficial factors, thereby improving composition consistency and manufacturability while retaining the necessary restorative activity.
Solution Approach 2:
The patent applies different processing conditions and purification methods to different components of the plasma to optimize their recovery and purity. By tailoring the processing approach to each specific factor's properties, the patent achieves high consistency in the final composition while maximizing the quantity of restorative factors.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively improves cellular health by reducing senescence-related gene expression, increasing telomere length, and enhancing immune and hematopoietic functions, leading to improved cellular viability and reduced inflammation.
Implementation Method 1
culturing the first cell sample in the presence of at least a portion of a culture media of the second cell sample for a time period ranging from about 24 hours to about 6 weeks to produce a restoring composition
Implementation Method 2
contacting the restoring composition with the second cell sample for a period of time ranging from about 24 hours to about 6 weeks to produce a restored composition
Implementation Method 3
a pharmaceutical formulation comprising an exosome isolated from a restoring composition
Data Source
AI summary
A method comprising obtaining a first cell sample from a first subject; obtaining a second cell sample from a second subject; culturing the first cell sample in the presence of at least a portion of a culture media of the second cell sample for a time period ranging from about 24 hours to about 6 weeks to produce a restoring composition; and contacting the restoring composition with the second cell sample for a period of time ranging from about 24 hours to about 6 weeks to produce a restored composition.