Cellular Spraying Compound for Skin Regeneration
Solution Overview
Problem
Current methods for skin regeneration, such as allogeneic grafts and bioprinting, face limitations due to immunological rejection, low availability of biological materials, susceptibility to infections, and incomplete regeneration of skin functionality, including pigmentation and hair induction, leading to prolonged recovery times and high public and private expenses.
Innovation Solution
A cellular spraying compound comprising microvascular endothelial cells and mesenchymal stem cells, pre-expanded in vitro and resuspended in a regenerative solution containing platelet-rich plasma, is applied via a medical aerosolization device, which promotes rapid re-epithelization and neovascularization by forming a polymerized gel layer that encapsulates and protects the cells, facilitating the regeneration of new skin tissue.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If allogeneic grafts and xeno transplants are used for skin regeneration, then the availability of biological material is improved, but immunological rejection and inflammatory processes increase
Solution Approach 1:
The patent extracts and isolates specific cell types (microvascular endothelial cells and mesenchymal stem cells) from donor tissue, separating them from the complex allogeneic graft system. This allows the use of purified cells that can be cultured and applied without the immunological rejection problems associated with whole organ or tissue grafts, while maintaining biological activity for skin regeneration.
Solution Approach 2:
The patent creates a simplified model system using cell culture techniques to reproduce functional skin regeneration capabilities. By culturing cells in vitro and applying them as a cellular spray composition, the patent creates a copy of the natural healing process that avoids the immunological complications of allogeneic grafts while maintaining regenerative function.
2Reliability
If traditional recovery methods are used for burn patients, then the body's natural healing process is activated, but fibrotic tissue formation and prolonged recovery time increase
Solution Approach 1:
The patent applies cellular components (microvascular endothelial cells and mesenchymal stem cells) to the wound site before the natural healing process can progress to fibrotic stages. This preliminary cellular intervention accelerates re-epithelization and neovascularization, preventing the body's natural response from progressing toward fibrosis and extending recovery time.
Solution Approach 2:
The patent introduces a cellular spray composition as an intermediary substance that mediates between the wound site and the body's natural healing mechanisms. This composition contains growth factors and cells that facilitate controlled regeneration, preventing uncontrolled fibrotic responses while accelerating the healing process.
3Quantity of substance
If mechanical deposition of autologous cells is used for cutaneous wounds, then the availability of suitable cells is improved, but the complexity of the application process increases
Solution Approach 1:
The patent employs aerosolization technology to deliver the cellular spray composition to the wound site. By using pneumatic spray devices, the complex cellular suspension can be applied uniformly and controllably to the wound surface, simplifying the application process compared to direct mechanical deposition methods while maintaining cell availability and viability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances skin regeneration by reducing inflammation, preventing fibrotic tissue formation, and promoting early angiogenesis, resulting in rapid tissue repair, reduced morbidity, and minimized risk of infection, while avoiding the formation of keloid scars and ensuring the recovery of skin functionality.
Implementation Method 1
a regenerative solution of cellular implantation composed of a combination of biocompatible and biodegradables biomaterials mainly containing a heme derived from a plasma rich in platelets (plasma rich in growth factors) and fibrinogen
Implementation Method 2
This effect brings as a result a rapid re-epithelization and regeneration of the cutaneous wound
Implementation Method 3
a heme derived from a plasma rich in platelets (plasma rich in growth factors) and fibrinogen that potentiate the therapeutic effect of the cells
Implementation Method 4
method of application by spraying and/or pulverizing without damaging the cells
Data Source
AI summary
The invention relates to a process for obtaining a cellular sprinkling compound and to the respective method of application to provide a therapeutic treatment for skin injuries, based on the implantation, by sprinkling and/or spraying, of human mesenchymal stem cells and microvascular endothelial cells that have been pre-expanded in vitro and resuspended in a regenerative solution for cellular implantation of biocompatible biomaterials. The solution is formed by blood plasma rich in growth factors obtained from the patient to be treated and, in some cases, by medical-grade type I collagen and by medical-grade hyaluronic acid, which potentiates the regeneration, re-epithelialization, and reconstruction of skin tissue.