Lateral Flow Assay Device for Centrifugal Sample Preparation
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Solution Overview
Problem
Lateral flow assays for solid materials require manual and time-consuming preparatory steps, which are prone to errors, especially when extracting and preparing samples from solid materials like cereal, necessitating trained laboratory technicians.
Innovation Solution
A device with a receptacle, compartment, and liquid passageway that allows for automated sample preparation by centrifugal force to transfer a filtered liquid sample upwards against gravity, ensuring even distribution and integration with a lateral flow test strip, optionally incorporating a sample well and container for chemical reactions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual preparatory steps are used for extracting and preparing samples from solid materials, then the process can be performed with simple equipment, but the process is time-consuming and error-prone requiring trained laboratory technicians
Solution Approach 1:
The patent combines multiple manual preparatory steps (extraction, filtration, sample transfer) into a single integrated automated device. The receptacle, filter, and liquid passageway are merged into one unit that performs extraction and sample delivery automatically, eliminating the need for separate manual operations and trained technicians.
Solution Approach 2:
The device enables self-service automation where the system performs sample preparation autonomously without human intervention. The centrifugal mechanism automatically transfers liquid samples from the receptacle through the filter to the test strip, and the analyser automatically detects results, replacing manual laboratory techniques.
2Loss of time
If automated sample preparation is implemented, then time consumption and errors are reduced, but the device complexity increases
Solution Approach 1:
The patent replaces complex mechanical automation systems with a simpler centrifugal-based mechanism. Instead of using pumps, valves, or complex actuation systems, the device uses centrifugal force generated by rotation to automatically transfer samples, achieving automation with minimal mechanical complexity.
Solution Approach 2:
The device changes the physical parameter of the sample from solid/mixed state to liquid phase through centrifugal separation. This parameter change enables automatic liquid flow through the filter and passageway to the test strip, facilitating automation without complex mechanical control systems.
3Manufacturing precision
If centrifugal force is used to transfer liquid sample upwards against gravity, then even distribution across the test strip is achieved, but the device complexity increases
Solution Approach 1:
The patent creates an equipotential distribution of the liquid sample across the test strip by using centrifugal force. The rotation generates uniform radial acceleration that distributes the liquid evenly across the absorbent strip width, achieving uniform sample application without complex positioning or dispensing mechanisms.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates automated and efficient sample preparation with reduced errors, enabling more even distribution and integration with a lateral flow assay, suitable for various applications including enzyme-linked immunosorbent assays (ELISA).
Implementation Method 1
The sample is carried towards the opposite short side of the test strip by mobile, typically liquid, phase that traverses the test strip, usually by capillary action (or 'wicking')
Implementation Method 2
A device with a receptacle, compartment, and liquid passageway that allows for automated sample preparation by centrifugal force to transfer a filtered liquid sample upwards against gravity
Data Source
AI summary
A device comprises: a receptacle having a bottom wall portion closing a first end of the receptacle to delimit an inner space and having a receptacle opening at an opposite second end; a compartment for holding a lateral flow assay test strip, the compartment having a lower portion located closer to the first end than to the second end; a passageway in fluid communication with the lower portion of the compartment; and a lid configured to overlay the receptacle opening and comprising a body portion. A sample receiving portion is located in said body portion and is arranged in fluid communication with the inner space. The liquid passageway is delimited by a first opening into the lower portion of the compartment and a second opening in the lid.