Centrifugal Cell Capture Device With Adhesion Layer
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Solution Overview
Problem
Conventional cell separation methods face difficulties in efficiently capturing target cells, such as circulating tumor cells, which are present in trace amounts in blood.
Innovation Solution
A centrifugal container with a cell adhesion layer made of a biocompatible polymeric material is used, where the layer is disposed in a capture region not parallel to the centrifugal direction, allowing for efficient capture of target cells through centrifugal force.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional cell separation method using wettable composition is used, then cells can be separated, but target cells present in trace amounts cannot be captured efficiently
Solution Approach 1:
The invention applies local quality by creating a cell adhesion layer with specific properties (biocompatible polymeric material containing intermediate water) at a specific location (capture region on the inner surface of the centrifugal container). This localized functional region enhances the capture efficiency for target cells in trace amounts without affecting the entire system, directly resolving the contradiction between capture efficiency and trace cell quantity.
Solution Approach 2:
The invention changes the physical and chemical parameters of the capture surface by using a biocompatible polymeric material that contains intermediate water. This parameter change (from conventional wettable composition to polymeric material with intermediate water) significantly improves the ability to capture target cells in trace amounts, addressing the productivity issue while accommodating the low quantity of target cells.
2Productivity
If cell adhesion layer is disposed parallel to centrifugal direction, then centrifugal force can be applied, but target cells cannot be captured efficiently
Solution Approach 1:
The invention applies asymmetry by orienting the cell adhesion layer at a specific angle (not parallel) to the centrifugal direction. This asymmetric orientation creates optimal conditions for target cell capture by allowing centrifugal force to drive cells toward the capture region while maintaining effective adhesion, thereby improving capture rate without requiring parallel alignment.
Solution Approach 2:
The invention introduces a dimensional aspect by considering the angular orientation of the cell adhesion layer relative to the centrifugal force vector. By optimizing this angular dimension, the system achieves efficient target cell capture that neither parallel nor perpendicular orientations could provide alone, resolving the contradiction between capture rate and layer orientation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enables efficient capture of target cells while minimizing damage, allowing them to be captured in a live state with high capture rates.
Implementation Method 1
a cell adhesion layer that is disposed on the inner surface and includes a first biocompatible polymeric material capable of containing intermediate water
Implementation Method 2
the first biocompatible polymeric material capable of containing intermediate water
Implementation Method 3
a centrifugal container that can be mounted in a centrifuge, and that has a space in which a test liquid containing target cells is held
Data Source
AI summary
A cell capture apparatus comprises a centrifugal container that can be mounted in a centrifuge, and that has a space in which a test liquid containing target cells is held, and an inner surface that defines the space; and a cell adhesion layer that is disposed on the inner surface and adsorbs the target cells in the test liquid.