Cerium Oxide Polymer Support for Nucleic Acid Collection
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Solution Overview
Problem
Current methods for collecting nucleic acids, such as the Boom method and those using iron oxide or silica gel, face challenges in efficiently adsorbing and eluting short-chain nucleic acids like pre-miRNAs and miRNAs due to low adsorption ratios and purity issues.
Innovation Solution
A support comprising a water-soluble neutral polymer adsorbed on a cerium oxide surface is used to enhance nucleic acid adsorption and elution efficiency, allowing for high-yield collection of nucleic acids ranging from short-chain to long-chain molecules.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If silica gel is used as a support for nucleic acid collection, then long-chain nucleic acids can be collected, but short-chain nucleic acids (pre-miRNAs and miRNAs of not more than 100 bases) exhibit low adsorption and cannot be collected efficiently
Solution Approach 1:
The invention changes the chemical parameters of the support surface by coating cerium oxide with a water-soluble neutral polymer. This parameter change transforms the surface properties to enable efficient adsorption of short-chain nucleic acids while maintaining the ability to collect long-chain nucleic acids, thus resolving the contradiction between collection yield and adaptability across different nucleic acid lengths
Solution Approach 2:
The invention creates a composite material structure by coating cerium oxide particles with a water-soluble neutral polymer. This composite structure combines the high relative gravity and stability of cerium oxide with the nucleic acid adsorption capabilities of the polymer coating, enabling efficient collection of both short-chain and long-chain nucleic acids
2Ease of operation
If iron oxide is used as a magnetic support for nucleic acid collection, then the collection process can be simplified, but inorganic components are eluted into the collecting solution, reducing nucleic acid purity
Solution Approach 1:
The water-soluble neutral polymer coating acts as an intermediary layer between the cerium oxide support and the nucleic acid. This intermediary coating prevents direct contact between the inorganic cerium oxide surface and the nucleic acid, thereby preventing inorganic component elution while maintaining ease of operation through magnetic separation
Solution Approach 2:
The water-soluble neutral polymer forms a thin film coating on the cerium oxide surface. This flexible polymer shell protects the nucleic acid from direct interaction with the inorganic support, preventing contamination while allowing the magnetic separation process to proceed efficiently
3Productivity
If titania or other high relative gravity particles are added to silica gel to increase column density, then plasmid collection efficiency is improved, but small fragment nucleic acids are not adsorbed on the support
Solution Approach 1:
The invention fundamentally changes the surface parameters of the support by coating cerium oxide with a water-soluble neutral polymer. This parameter change creates a surface chemistry that is highly effective for adsorbing small fragment nucleic acids while maintaining high collection efficiency for plasmids, thus resolving the contradiction between productivity and adaptability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables high-yield collection of nucleic acids, including short-chain pre-miRNAs and miRNAs, with improved adsorption and elution ratios, overcoming the limitations of existing techniques.
Implementation Method 1
a support comprising a water-soluble neutral polymer adsorbed on a cerium oxide surface thereof
Implementation Method 2
the fact that nucleic acids in the form of small fragments are not adsorbed on a support is described in the paragraph [0134]
Data Source
AI summary
A method of collecting a nucleic acid from a biological sample, comprising the steps of: a) mixing a support comprising a water-soluble neutral polymer adsorbed on a cerium oxide surface thereof with a solution containing a nucleic acid to adsorb the nucleic acid to the support, b) separating the support on which the nucleic acid was adsorbed from the solution mixed in the step a), and c) collecting the nucleic acid by adding an eluent to the support on which the nucleic acid was adsorbed and which is separated in the step b).