Hybrid Corn CH100489 Cytoplasmic Male Sterility Breeding

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH100489, which incorporates genetic modifications and cytoplasmic male sterility to prevent self-pollination, along with specific traits like herbicide resistance and disease resistance, achieved through genetic transformation and backcrossing techniques, allowing for controlled cross-pollination and stable hybrid seed production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If physical emasculation is used to prevent self-pollination, then cross-pollination control is improved, but labor intensity and cost increase

Engineering Contradiction:
Improvecross-pollination controlVSAvoidbreeding efficiency
Core Design Contradiction:
ReliabilityVSEase of manufacture

Solution Approach 1:

The patent replaces mechanical emasculation with a biological system - cytoplasmic male sterility (CMS). The CMS trait genetically prevents pollen production in the female parent, eliminating the need for manual or mechanical removal of male parts. This biological substitution maintains reliable cross-pollination control while dramatically reducing labor intensity and breeding costs.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Adaptability or versatility

If traditional breeding methods are used to develop hybrid varieties, then genetic diversity is improved, but time required for variety development increases

Engineering Contradiction:
Improvegenetic diversityVSAvoidbreeding cycle duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-developing and maintaining CMS inbred lines before hybrid production. The CMS female parent is prepared in advance with confirmed male sterility, and suitable restorer lines are pre-identified. This preliminary preparation eliminates time-consuming emasculation operations during the actual hybrid seed production season, significantly accelerating the breeding cycle while maintaining genetic diversity through careful selection of parental lines.

Inventive Principle:
Principle #10Preliminary action

3Quantity of substance

If self-pollination is allowed in hybrid production, then seed set is improved, but genetic purity of hybrid decreases

Engineering Contradiction:
Improveseed setVSAvoidgenetic purity
Core Design Contradiction:
Quantity of substanceVSStability of the object's composition

Solution Approach 1:

The patent introduces a genetic intermediary - the CMS restorer gene - that mediates between seed set requirements and genetic purity. The female parent carries CMS to prevent self-pollination and ensure genetic purity. The male parent carries the restorer gene that restores fertility in the F1 hybrid progeny. This intermediary system allows complete prevention of self-pollination during parent generation while ensuring high seed set in the hybrid generation through restored fertility.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS11013194B2Plants and seeds of hybrid corn variety CH100489
Publication Date: 2021.05.25 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH100489. The invention thus relates to the plants, seeds and tissue cultures of the variety CH100489, and to methods for producing a corn plant produced by crossing a corn plant of variety CH100489 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH100489.