Hybrid Corn CH416403 Cytoplasmic Male Sterility Breeding

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH416403, which incorporates genetic modifications and cytoplasmic male sterility traits to prevent self-pollination, along with specific loci conferring herbicide resistance, disease resistance, and other desirable characteristics, using advanced breeding methods and genetic transformation techniques like CRISPR-Cas systems.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional self-pollination breeding methods are used, then genetic uniformity can be maintained, but hybrid vigor and yield improvement are limited

Engineering Contradiction:
ImproveyieldVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The breeding program segments the population into distinct inbred lines through repeated self-pollination and selection, creating genetically uniform parental lines that can be crossed to produce hybrids with heterosis while maintaining overall genetic stability through controlled mating designs

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent combines desirable traits from multiple inbred parental lines into a single hybrid variety through controlled cross-pollination, merging genetic material from parents with different advantageous characteristics to achieve superior yield and performance

Inventive Principle:
Principle #5Merging (Combining)

2Productivity

If cross-pollination is used to develop hybrid varieties, then hybrid vigor and yield are improved, but self-pollination control and genetic purity become challenging

Engineering Contradiction:
ImproveyieldVSAvoidgenetic purity
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent extracts and eliminates the capability for self-pollination by introducing cytoplasmic male sterility, removing the problematic self-fertilization pathway while preserving the desired cross-pollination mechanism for hybrid seed production

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent uses sterile cytoplasm as an intermediary mechanism to prevent self-pollination, where the cytoplasmic factors act as a biological mediator that blocks pollen function in male-sterile lines, ensuring genetic purity without requiring physical isolation

Inventive Principle:
Principle #24Intermediary (Mediator)

3Productivity

If male sterility systems are introduced to prevent self-pollination, then hybrid seed production efficiency is improved, but system complexity and breeding difficulty increase

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidbreeding system complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The breeding system uses self-service mechanisms where the cytoplasmic male sterility automatically prevents self-pollination without requiring external intervention, and the restorer genes in the male parent automatically restore fertility in the F1 hybrid, eliminating the need for manual emasculation or complex management procedures

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS20210000056A1Plants and seeds of hybrid corn variety ch416403
Publication Date: 2021.01.07 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH416403. The invention thus relates to the plants, seeds and tissue cultures of the variety CH416403, and to methods for producing a corn plant produced by crossing a corn plant of variety CH416403 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH416403.