Hybrid Corn CH968225 Cytoplasmic Male Sterility Seed Production

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH968225, which incorporates genetic modifications and cytoplasmic male sterility traits to prevent self-pollination, along with tissue culture methods for regenerating plants with specific physiological and morphological characteristics, enables the production of seeds with enhanced traits like herbicide resistance and improved agronomic qualities.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional self-pollination breeding methods are used, then genetic stability is maintained, but hybrid seed production efficiency is limited due to self-pollination

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent applies preliminary anti-action by introducing cytoplasmic male sterility traits that prevent self-pollination before it can occur. The sterile cytoplasm is transferred to female parent lines through backcrossing, creating plants that cannot self-pollinate and must receive cross-pollen, thereby ensuring hybrid seed production while maintaining genetic control through the use of maintainer and restorer lines.

Inventive Principle:
Principle #9Preliminary anti-action

Solution Approach 2:

The patent uses intermediary elements in the form of maintainer lines and restorer lines that mediate between the sterile cytoplasm and fertile phenotypes. The maintainer line preserves the sterile cytoplasm without restoring fertility, while the restorer line introduces nuclear genes that restore male fertility in the hybrid, enabling controlled cross-pollination for hybrid seed production.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If cytoplasmic male sterility traits are introduced to prevent self-pollination, then hybrid seed production efficiency is improved, but device complexity increases due to breeding program requirements

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidbreeding program complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent segments the breeding program into distinct functional components: sterile cytoplasm transfer through backcrossing to create female parent lines, maintenance of sterile cytoplasm through maintainer lines, and restoration of fertility through restorer lines. This segmentation allows each component to be developed and maintained independently, simplifying the overall breeding program despite the complexity of preventing self-pollination.

Inventive Principle:
Principle #1Segmentation

3Adaptability or versatility

If multiple desirable traits are combined in a single hybrid variety, then agronomic quality is improved, but breeding program duration increases

Engineering Contradiction:
Improveagronomic qualityVSAvoidbreeding program duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-transferring the sterile cytoplasm to the female parent line through backcrossing before the final hybridization step. This preliminary establishment of the sterile cytoplasm foundation allows subsequent crossing with restorer lines to simultaneously introduce multiple desirable traits without requiring prolonged breeding programs, as the sterility mechanism is already in place.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS20200396924A1Plants and seeds of hybrid corn variety ch968225
Publication Date: 2020.12.24 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH968225. The invention thus relates to the plants, seeds and tissue cultures of the variety CH968225, and to methods for producing a corn plant produced by crossing a corn plant of variety CH968225 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH968225.