Chemiluminescence Detection With Wavelength-Conversion Coating
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing chemiluminescence detection systems suffer from reduced sensitivity due to a mismatch between the luminescent emission wavelengths of chemiluminescent tags and the maximum quantum efficiency of detectors, leading to significant losses in photon flux and detection sensitivity, particularly when using red dye labels.
Innovation Solution
A luminescence detection system with a conversion coating that shifts luminescent emissions to align with the maximum detection efficiency wavelength range of the detector, allowing for higher photon flux and improved sensitivity by converting photons outside the optimal range to fall within it.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Illumination intensity
If red dye labels are used to increase quantum yield, then the luminescent emission intensity is improved, but the detection sensitivity deteriorates due to mismatch with detector maximum quantum efficiency wavelength range
Solution Approach 1:
A wavelength conversion coating is introduced as an intermediary between the red dye label and the detector. This coating converts the luminescent emissions from red dye labels (which have high quantum yield but mismatched wavelength) into wavelengths that fall within the maximum quantum efficiency range of the detector, thereby resolving the contradiction between emission intensity and detection sensitivity
Solution Approach 2:
The patent changes the wavelength parameter of the luminescent emissions through the wavelength conversion coating. By transforming the emission wavelength from the red dye label's peak (which is outside the detector's optimal range) to a wavelength within the detector's maximum quantum efficiency range, the system achieves both high intensity detection and high sensitivity
2Quantity of substance
If chemiluminescent tags with high quantum yield are used, then the photon flux is improved, but the detection sensitivity deteriorates due to wavelength mismatch with the detector
Solution Approach 1:
The wavelength conversion coating acts as a mediator that bridges the gap between high quantum yield chemiluminescent tags and the detector. It converts the photons from tags with high photon flux but mismatched wavelength into photons within the detector's optimal wavelength range, thereby maintaining both high photon flux and high detection sensitivity
3Measurement precision
If the detector is optimized for a specific wavelength range, then the detection efficiency is improved, but the adaptability to different chemiluminescent tags deteriorates
Solution Approach 1:
The wavelength conversion coating provides universality by enabling the detector to efficiently detect different chemiluminescent tags with varying emission wavelengths. The coating converts emissions from various tags (e.g., red dye labels, acridinium esters) into the detector's optimal wavelength range, making the detection system adaptable to multiple tag types while maintaining high detection efficiency for each
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system enhances the overall signal strength and sensitivity of luminescence detection by effectively utilizing higher quantum yield labels, such as red dye labels, and minimizes intensity loss, thereby improving the detection of luminescent emissions.
Implementation Method 1
A conversion coating (122) is provided at a location adjacent to the light entrance window (120). The conversion coating (122) is designed to convert the luminescent emissions (116) to incident emissions (126) that have been shifted in wavelength to fall within a second wavelength range (228) having an incident peak (230) lying within the maximum detection efficiency wavelength range (224)
Implementation Method 2
a photodetector (118), such as a photomultiplier tube (PMT), having a light entrance window (120) and a maximum detection efficiency wavelength range (224)
Implementation Method 3
Chemiluminescence (CL) is defined as the emission of electromagnetic radiation caused by a chemical reaction to produce light
Data Source
Figure 1A~1B
Figure 1C
Figure 2A~2B
AI summary
A luminescence detection system for use in immunoassay testing. Luminescence detection system comprises a sample holder configured to hold a test sample including labeled components, wherein the labeled components in the test sample undergo a chemiluminescent reaction and emit luminescent emissions over a first wavelength range, a photodetector having a light entrance window configured to receive light emissions, the photodetector having a maximum detection efficiency wavelength range, and a conversion member provided adjacent the light entrance window that operates to cause conversion of the luminescent emissions over the first wavelength range to incident emissions of a second wavelength range wherein an incident peak of the incident emissions falls within the maximum detection efficiency wavelength range where the quantum efficiency of the photodetector is 10% or more. Methods of luminescence detection are provided, as are other aspects.