Chemiluminescent Assay Background Correction for Interference

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Solution Overview

Problem

Existing chemiluminescence-based assays face interference from compounds other than the analyte, leading to inaccurate analyte concentration measurements, particularly in complex samples like food substances, due to interference with oxidation or reduction reactions, which is difficult to avoid and significantly diluting the sample reduces the lowest level of detection and quantification.

Innovation Solution

Incorporating a chemiluminescent substrate as a background agent that produces a luminescent background signal independently of analyte binding, allowing for a scale factor correction by comparing the background signal ratio between test and standard samples to accurately determine analyte concentration.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If chemiluminescence-based assays are used to detect analyte concentration, then detection sensitivity is improved, but measurement precision deteriorates due to interference from non-analyte compounds

Engineering Contradiction:

Improveanalyte concentration measurement accuracyVSAvoidinterference from non-analyte compounds

Core Design Contradiction:

Measurement precisionVSObject-affected harmful factors

Solution Approach 1:

The patent introduces a trigger solution as an intermediary substance that mediates the chemiluminescent reaction. This trigger solution contains components that selectively interact with the analyte-bound chemiluminescent-labeled specific binding partner to produce a measurable signal, while being unaffected by interfering compounds. The trigger solution acts as a mediator that converts the binding event into a controlled chemical reaction, enabling precise measurement despite the presence of interferents in the sample.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent extracts the harmful interference effect by separating the signal generation step from the binding step. By using a trigger solution that only activates the chemiluminescent label after analyte binding has occurred, the method extracts the analyte-specific signal from the background noise caused by interfering compounds. The trigger solution selectively activates only the analyte-bound complex, effectively removing interfering substances from the signal measurement.

Inventive Principle:

Principle #2Taking out (Extraction)

2Measurement precision

If chemiluminescent substrate is added to produce background signal, then measurement precision is improved through correction factor, but device complexity increases

Engineering Contradiction:

Improveanalyte concentration measurement accuracyVSAvoidassay system complexity

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent merges the background signal generation and analyte signal generation into a single chemiluminescent reaction system. The chemiluminescent substrate serves dual purposes: it provides the background signal for correction and participates in the analyte detection signal. By combining these functions into one integrated system rather than using separate detection mechanisms, the patent reduces overall system complexity while maintaining measurement precision through the correction factor approach.

Inventive Principle:

Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method provides accurate analyte concentration measurements by correcting for interference, maintaining low detection and quantification levels without significant sample dilution, applicable to various binding agents and formats.

Implementation Method 1

the trigger solution is capable of producing a background signal from the unbound chemiluminescent substrate

Methodology Applied

Scientific EffectChemiluminescence: Chemiluminescence

Implementation Method 2

the chemiluminescent-labeled specific bonding partner being capable of binding to the analyte to form an analyte-bound chemiluminescent labeled specific binding complex

Methodology Applied

Scientific EffectSpecific binding: