Chimeric Adenovirus DSG2 Targeting for CTC Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for detecting circulating tumor cells (CTCs) are inefficient due to their low numbers in blood and the invasive nature of traditional tissue biopsies, which are costly and painful.
Innovation Solution
A chimeric adenovirus, specifically designed with a knob domain protein of adenovirus type 3, is used to target DSG2, enabling the detection and isolation of CTCs from blood samples without invasive procedures.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If tissue biopsy is used for cancer diagnosis, then genetic traits can be collected for personalized treatment, but the procedure is invasive, costly, and painful for patients
Solution Approach 1:
The invention extracts the essential function of tissue biopsy (collecting genetic material from cancer cells) and separates it from the invasive procedure by detecting cancer-specific genetic markers in circulating tumor cells that naturally shed into the bloodstream, eliminating the need for invasive tissue sampling
Solution Approach 2:
The invention uses circulating tumor cells in blood as an intermediary medium to obtain cancer genetic information without directly accessing the primary tumor site through invasive procedures. The blood serves as a non-invasive intermediary that contains shed cancer cells with the required genetic material
2Measurement precision
If conventional detection methods are used for CTCs, then detection can be performed, but the low number of CTCs (one out of 10^9 red blood cells) makes accurate detection extremely difficult
Solution Approach 1:
The invention employs fluorescent labeling that causes CTCs to emit specific colors or fluorescence signals when bound by antibodies, enabling visual differentiation and precise detection of CTCs against the background of billions of non-fluorescent red blood cells
Solution Approach 2:
The invention changes the detectable parameters of CTCs by coating them with fluorescently labeled antibodies, transforming them from undetectable cells to cells with distinct optical signatures that can be easily identified and counted using flow cytometry or fluorescence microscopy
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The adenovirus effectively targets and isolates CTCs, allowing for early cancer diagnosis, analysis of primary cancer type and stage, prediction of cancer prognosis, and monitoring of cancer progression and drug response, thereby facilitating personalized treatment.
Implementation Method 1
the adenovirus includes: a human telomere promoter (hTERT) operably linked to E1A and E1B of an endogenous gene of the adenovirus; a marker gene inserted into an E3 region of the endogenous gene of the adenovirus; and a knob domain protein of adenovirus type 3
Data Source
AI summary
The present disclosure relates to a chimeric adenovirus for detecting circulating tumor cells. An adenovirus type 5/3 for detecting circulating tumor cells, according to the present disclosure, comprises a knob domain of adenovirus type 3 and thus targets DSG2, and thus can also detect EMT circulating tumor cells, has excellent viability in the blood, and can accurately detect and isolate circulating tumor cells from more carcinomas, and thus enables early diagnosis of cancer and analysis of the type and stage of primary cancer, and can predict cancer prognosis, monitor cancer progression and analyze drug response and therapeutic effects through the genetic analysis of isolated circulating tumor cells, and therefore can be effectively used in personalized treatment.