Chimeric Lysozyme Variant for Antibiotic-Free Animal Feed
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Solution Overview
Problem
The application of lysozyme in animal feed is limited due to high extraction costs and poor thermal stability, which hinders its effectiveness in high-temperature granulation processes and productivity.
Innovation Solution
A chimeric lysozyme variant with improved heat resistance is developed through microbial fermentation, achieving nearly double the thermal stability at 60°C and high-efficiency expression in host cells, which is then used in animal feed additives to enhance growth performance and feed conversion rates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If lysozyme is extracted from egg white, then lysozyme can be obtained for use in animal feed, but the productivity is low and costs are high
Solution Approach 1:
The patent replaces expensive egg white extraction with microbial fermentation using inexpensive culture media, achieving lower cost production. The microbial system serves as a disposable, renewable source of lysozyme that does not depend on animal byproducts.
Solution Approach 2:
The patent modifies the source and production parameters of lysozyme by switching from egg white extraction to microbial fermentation systems, fundamentally changing the manufacturing approach to improve both cost and productivity simultaneously.
2Reliability
If traditional lysozyme is used in animal feed, then antibacterial effects are achieved, but thermal stability is poor and cannot meet high-temperature granulation requirements
Solution Approach 1:
The patent changes the physical-chemical parameters of lysozyme by selecting thermally stable variants and optimizing fermentation conditions to produce lysozyme with enhanced thermal resistance, enabling survival through high-temperature granulation processes.
Solution Approach 2:
The patent uses microbial fermentation to produce disposable, single-use lysozyme preparations that are optimized for specific thermal stability requirements, replacing the need for expensive, thermally unstable natural extracts.
3Productivity
If antibiotics are used in animal feed, then growth promotion effects are achieved, but drug resistance develops and affects human health
Solution Approach 1:
The patent converts the harmful overuse of antibiotics into a beneficial alternative by using lysozyme, a natural enzyme with selective antibacterial activity that targets pathogenic bacteria without promoting resistance, thus maintaining growth promotion benefits while eliminating harmful side effects.
Solution Approach 2:
The patent changes the type of antimicrobial agent from antibiotics to lysozyme enzyme, fundamentally altering the mechanism of action from broad-spectrum bacterial inhibition to targeted cell wall hydrolysis, which does not induce resistance.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The chimeric lysozyme variant significantly improves animal growth performance and feed utilization rates, providing a safe and effective alternative to antibiotics while maintaining thermal stability, thus addressing the limitations of traditional lysozyme use in animal feeds.
Implementation Method 1
Lysozyme (EC 3.2.1.17) is a hydrolytic enzyme acting on the cell wall of microorganisms... It can effectively hydrolyze the peptidoglycan of the bacterial cell wall, which is mainly achieved by the mechanism of hydrolysis of the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine
Implementation Method 2
The thermal stability of the chimeric lysozyme variant under the condition of 60° C. is nearly doubled compared to a parental sequence
Data Source
AI summary
A chimeric lysozyme variant has an improved heat resistance and is encoded by a polynucleotide. A novel chimeric lysozyme sequence is constructed, and high-efficiency expression in a host cell is achieved. The thermal stability of the chimeric lysozyme under the condition of 60° C. is nearly doubled compared to a parental sequence. The chimeric lysozyme variant can be used to prepare product that is antibiotic, for example, animal feed additive containing the chimeric lysozyme variant and an animal feed containing the animal feed additive.