Analysis Chip Photometry Correction Using an In-Field Color Plate
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Solution Overview
Problem
Existing analysis apparatuses face inaccuracies in quantitative analysis due to variations in illuminance distribution from light sources, which are influenced by individual differences, installation errors, and light source deterioration.
Innovation Solution
The apparatus includes a support section for an analysis chip with a photometry unit that uses a color plate within the imaging range, along with a processor to correct measurement values based on a correlation between the reaction region brightness and the color plate brightness, derived from pre-photometric and pre-correction brightness values.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a reflective piece is placed within the visual field of the detector to correct reflectivity calculation errors, then measurement precision is improved, but the device complexity increases due to additional components and calibration procedures
Solution Approach 1:
The patent introduces a color plate as an intermediary reference standard that reflects light with known optical properties. This color plate serves as a mediator between the light source and the reaction region, enabling the system to measure and correct for illuminance distribution variations without requiring complex multi-component reference systems. The color plate's standardized reflectivity characteristics allow for precise correction calculations while simplifying the overall system architecture.
Solution Approach 2:
The patent utilizes the relationship between the brightness values of the color plate and the reaction region to establish correction parameters. By measuring the brightness of the color plate (which has known optical density) and comparing it with the brightness of the reaction region, the system calculates correction factors that compensate for illuminance distribution variations. This parameter-based approach enables precise measurement correction through mathematical relationships rather than complex hardware modifications.
2Measurement precision
If the light source illuminance distribution varies due to individual differences, installation errors, and deterioration, then measurement precision deteriorates, but adding correction mechanisms increases device complexity
Solution Approach 1:
The patent implements a feedback mechanism where the brightness value of the color plate is measured and used to calculate correction factors that are applied to the reaction region measurements. This feedback loop continuously compensates for illuminance distribution variations, ensuring that measurement precision is maintained despite changes in light source performance. The system uses the color plate's known optical properties as a reference to detect and correct deviations in real-time.
Solution Approach 2:
The color plate acts as a stable intermediary reference that does not deteriorate like the light source. By measuring the color plate's brightness and using it to calculate correction factors, the system transfers the stability of the color plate's optical properties to the measurement of the reaction region. This intermediary approach allows the system to compensate for light source deterioration without requiring the light source itself to be replaced or recalibrated.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables high-accuracy quantitative analysis by correcting for illuminance distribution variations, ensuring precise measurement of detection target substances.
Implementation Method 1
a light-emitting element that irradiates the reaction region of the analysis chip with measurement light
Implementation Method 2
detecting reflected light thereof
Data Source
Figure 1
Figure 2
Figure 3~4
AI summary
An analysis apparatus (100) includes: a support section (62) that supports an analysis chip (12) at a measurement position; a photometry unit (70) including a light-emitting element (73a, 73b) that irradiates a reaction region of the analysis chip (12) with measurement light, and an area sensor (74) that captures an image of a predetermined imaging range including the reaction region irradiated with the measurement light; a color plate (75) arranged within the imaging range and having a region irradiated with the measurement light; and a processor (90) that performs quantitative analysis of a detection target substance based on a measurement value corresponding to a photometric region brightness value, which is a brightness value of the reaction region extracted from an image acquired from the photometry unit, and that extracts a correction brightness value, which is a brightness value of the color plate, from the image in addition to the photometric region brightness value to correct the measurement value based on the correction brightness value and a correlation between a pre-photometric region brightness value, which is a brightness value of a region corresponding to the reaction region and acquired in advance using a reference plate, and a pre-correction brightness value which is a brightness value of the color plate.